Difference between revisions of "LibraryPrep"
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=== Watchmaker for mRNA === | === Watchmaker for mRNA === | ||
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA. | For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA. | ||
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=== Watchmaker for Ribodepletion === | === Watchmaker for Ribodepletion === | ||
Revision as of 17:08, 10 July 2024
Contents
- 1 Library prep
- 1.1 RNA
- 1.1.1 Watchmaker for mRNA
- 1.1.2 Watchmaker for Ribodepletion
- 1.1.3 NEBnext small RNA
- 1.1.4 Clonetech SMART-Seq v4 Ultra-low
- 1.1.5 IDT/xGen for mRNA (Supported Only For Legacy Projects)
- 1.1.6 KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
- 1.1.7 KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
- 1.1.8 Clonetech SMARTer PICO (Supported Only For Legacy Projects)
- 1.2 DNA
- 1.3 Frequently Asked Questions
- 1.1 RNA
Library prep
RNA
Watchmaker for mRNA
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
Watchmaker for Ribodepletion
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis.
NEBnext small RNA
For microRNA and other small RNA. 100-1000ng total RNA.
Clonetech SMART-Seq v4 Ultra-low
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.
IDT/xGen for mRNA (Supported Only For Legacy Projects)
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.
KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)
Clonetech SMARTer PICO (Supported Only For Legacy Projects)
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.
DNA
Nextera gDNA
“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
Frequently Asked Questions
Please refer to frequently asked questions section for FAQ regarding library prep options.
