Difference between revisions of "LibraryPrep"
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= Library prep = | = Library prep = | ||
== RNA == | == RNA == | ||
| − | === KAPA Hyperprep mRNA === | + | === KAPA Hyperprep mRNA (Supported Only For Legacy Projects) === |
The most common RNA kit option. Best for standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA. | The most common RNA kit option. Best for standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA. | ||
| − | === KAPA Hyperprep with Ribo-depletion === | + | === KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects) === |
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better) | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better) | ||
| − | === Clonetech SMART-Seq v4 Ultra-low === | + | === Clonetech SMART-Seq v4 Ultra-low (Supported Only For Legacy Projects) === |
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA. | For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA. | ||
| − | === Clonetech SMARTer PICO === | + | === Clonetech SMARTer PICO (Supported Only For Legacy Projects) === |
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA. | For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA. | ||
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== DNA == | == DNA == | ||
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=== Nextera gDNA === | === Nextera gDNA === | ||
Revision as of 16:58, 10 July 2024
Contents
- 1 Library prep
- 1.1 RNA
- 1.1.1 KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
- 1.1.2 KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
- 1.1.3 Clonetech SMART-Seq v4 Ultra-low (Supported Only For Legacy Projects)
- 1.1.4 Clonetech SMARTer PICO (Supported Only For Legacy Projects)
- 1.1.5 NEBnext small RNA
- 1.2 DNA
- 1.3 Frequently Asked Questions
- 1.1 RNA
Library prep
RNA
KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
The most common RNA kit option. Best for standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.
KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)
Clonetech SMART-Seq v4 Ultra-low (Supported Only For Legacy Projects)
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.
Clonetech SMARTer PICO (Supported Only For Legacy Projects)
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.
NEBnext small RNA
For microRNA and other small RNA. 100-1000ng total RNA.
DNA
Nextera gDNA
“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
Frequently Asked Questions
Please refer to frequently asked questions section for FAQ regarding library prep options.
