Difference between revisions of "Equipment Resources"
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== QPCR System == | == QPCR System == | ||
| − | ABI 7700 Real-Time PCR System | + | ABI 7700 Real-Time PCR System: This system combines thermal cycling, fluorescence detection, and application-specific software to provide an integrated platform for the detection and quantification of nucleic acid sequences. Real-time PCR reactions run using 96-well plates, 384-well plates and TILDA cards and can be completed in under two hours. <font color=blue>Access available to non-cagt members</font> |
== Analytical Instruments for Sample Quality Assessment == | == Analytical Instruments for Sample Quality Assessment == | ||
| − | NanoDrop ND-1000(s): We now have two of these extremely popular and sensitive spectrophotometers to measure nucleic acid (DNA and RNA) and protein concentrations, calculate microarray fluorescent dye concentrations and determine cell densities. The nanoDrop 1000 produces a full spectrum analysis from 1-2 microliters of undiluted samples, and is completed in 10 seconds. | + | NanoDrop ND-1000(s): We now have two of these extremely popular and sensitive spectrophotometers to measure nucleic acid (DNA and RNA) and protein concentrations, calculate microarray fluorescent dye concentrations and determine cell densities. The nanoDrop 1000 produces a full spectrum analysis from 1-2 microliters of undiluted samples, and is completed in 10 seconds. <font color=blue>Access available to non-cagt members</font> |
== Analysis workstation == | == Analysis workstation == | ||
| − | CAGT provides this analysis workstation for data analysis. They are loaded with software for microarray data analysis and qPCR data analysis. Software packages installed include Axon's GenePix Pro, Cluster, Acuity, TreeView and RQ Manager. | + | CAGT provides this analysis workstation for data analysis. They are loaded with software for microarray data analysis and qPCR data analysis. Software packages installed include Axon's GenePix Pro, Cluster, Acuity, TreeView and RQ Manager. <font color=blue>Access available to non-cagt members</font> |
== Liquid Handling Robot == | == Liquid Handling Robot == | ||
| − | Beckman Biomek FX: To help out with repetitive pipettng tasks and manipulation of libraries that accompanies microarray production, a Beckman Biomek FX automation station is available to researchers. | + | Beckman Biomek FX: To help out with repetitive pipettng tasks and manipulation of libraries that accompanies microarray production, a Beckman Biomek FX automation station is available to researchers. <font color=blue>Access </font><font color=red>NOT</font> <font color=blue>available to non-cagt members</font> |
== Arrayers for Microarray Printing == | == Arrayers for Microarray Printing == | ||
| − | OmniGrid100: The OmniGrid 100 microarrayer can print biological samples on standard sized glass slides, 100 slides at a time. Printing is from microtiter plates with 96 or 384 wells. A server arm allows up to 72 source plates to be used. Features are printed as 100 to 200 micron spots, size depending on tip type used. The positioning system is high speed and uses high-reliability brushless DC servo motors. The instrument is enclosed in a humidity controlled chamber. | + | OmniGrid100: The OmniGrid 100 microarrayer can print biological samples on standard sized glass slides, 100 slides at a time. Printing is from microtiter plates with 96 or 384 wells. A server arm allows up to 72 source plates to be used. Features are printed as 100 to 200 micron spots, size depending on tip type used. The positioning system is high speed and uses high-reliability brushless DC servo motors. The instrument is enclosed in a humidity controlled chamber. <font color=blue>Access </font><font color=red>NOT</font> <font color=blue>available to non-cagt members</font> |
| − | Cartesian Technologies: The Cartesian system can hold up to 32 micro spotting pins, 50 slides and up to 50, 384-well microtiter plates providing the capacity to produce high-density microarrays with more than 20,000 DNA spots per slide. The PixSys 5500 is a multi-axis microarrayer that prints biological samples onto 25mm x 75mm glass substrates. | + | Cartesian Technologies: The Cartesian system can hold up to 32 micro spotting pins, 50 slides and up to 50, 384-well microtiter plates providing the capacity to produce high-density microarrays with more than 20,000 DNA spots per slide. The PixSys 5500 is a multi-axis microarrayer that prints biological samples onto 25mm x 75mm glass substrates. <font color=blue>Access </font><font color=red>NOT</font> <font color=blue>available to non-cagt members</font> |
Revision as of 11:33, 1 May 2008
Contents
QPCR System
ABI 7700 Real-Time PCR System: This system combines thermal cycling, fluorescence detection, and application-specific software to provide an integrated platform for the detection and quantification of nucleic acid sequences. Real-time PCR reactions run using 96-well plates, 384-well plates and TILDA cards and can be completed in under two hours. Access available to non-cagt members
Analytical Instruments for Sample Quality Assessment
NanoDrop ND-1000(s): We now have two of these extremely popular and sensitive spectrophotometers to measure nucleic acid (DNA and RNA) and protein concentrations, calculate microarray fluorescent dye concentrations and determine cell densities. The nanoDrop 1000 produces a full spectrum analysis from 1-2 microliters of undiluted samples, and is completed in 10 seconds. Access available to non-cagt members
Analysis workstation
CAGT provides this analysis workstation for data analysis. They are loaded with software for microarray data analysis and qPCR data analysis. Software packages installed include Axon's GenePix Pro, Cluster, Acuity, TreeView and RQ Manager. Access available to non-cagt members
Liquid Handling Robot
Beckman Biomek FX: To help out with repetitive pipettng tasks and manipulation of libraries that accompanies microarray production, a Beckman Biomek FX automation station is available to researchers. Access NOT available to non-cagt members
Arrayers for Microarray Printing
OmniGrid100: The OmniGrid 100 microarrayer can print biological samples on standard sized glass slides, 100 slides at a time. Printing is from microtiter plates with 96 or 384 wells. A server arm allows up to 72 source plates to be used. Features are printed as 100 to 200 micron spots, size depending on tip type used. The positioning system is high speed and uses high-reliability brushless DC servo motors. The instrument is enclosed in a humidity controlled chamber. Access NOT available to non-cagt members
Cartesian Technologies: The Cartesian system can hold up to 32 micro spotting pins, 50 slides and up to 50, 384-well microtiter plates providing the capacity to produce high-density microarrays with more than 20,000 DNA spots per slide. The PixSys 5500 is a multi-axis microarrayer that prints biological samples onto 25mm x 75mm glass substrates. Access NOT available to non-cagt members
