Difference between revisions of "LibraryPrep"
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= Library prep = | = Library prep = | ||
== RNA == | == RNA == | ||
| + | === Watchmaker for mRNA === | ||
| + | For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA. | ||
| + | |||
| + | === Watchmaker for Ribodepletion === | ||
| + | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. | ||
| + | |||
| + | === Watchmaker for Ribodepletion === | ||
| + | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. | ||
| + | |||
| + | === NEBnext small RNA === | ||
| + | For microRNA and other small RNA. 100-1000ng total RNA. | ||
| + | |||
| + | === Clonetech SMART-Seq v4 Ultra-low === | ||
| + | For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA. | ||
| + | |||
| + | === IDT/xGen for mRNA (Supported Only For Legacy Projects) === | ||
| + | For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA. | ||
| + | |||
=== KAPA Hyperprep mRNA (Supported Only For Legacy Projects) === | === KAPA Hyperprep mRNA (Supported Only For Legacy Projects) === | ||
| − | + | For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA. | |
=== KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects) === | === KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects) === | ||
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better) | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better) | ||
| − | |||
| − | |||
| − | |||
=== Clonetech SMARTer PICO (Supported Only For Legacy Projects) === | === Clonetech SMARTer PICO (Supported Only For Legacy Projects) === | ||
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA. | For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA. | ||
| − | |||
| − | |||
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== DNA == | == DNA == | ||
Revision as of 17:07, 10 July 2024
Contents
- 1 Library prep
- 1.1 RNA
- 1.1.1 Watchmaker for mRNA
- 1.1.2 Watchmaker for Ribodepletion
- 1.1.3 Watchmaker for Ribodepletion
- 1.1.4 NEBnext small RNA
- 1.1.5 Clonetech SMART-Seq v4 Ultra-low
- 1.1.6 IDT/xGen for mRNA (Supported Only For Legacy Projects)
- 1.1.7 KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
- 1.1.8 KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
- 1.1.9 Clonetech SMARTer PICO (Supported Only For Legacy Projects)
- 1.2 DNA
- 1.3 Frequently Asked Questions
- 1.1 RNA
Library prep
RNA
Watchmaker for mRNA
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
Watchmaker for Ribodepletion
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis.
Watchmaker for Ribodepletion
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis.
NEBnext small RNA
For microRNA and other small RNA. 100-1000ng total RNA.
Clonetech SMART-Seq v4 Ultra-low
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.
IDT/xGen for mRNA (Supported Only For Legacy Projects)
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.
KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)
Clonetech SMARTer PICO (Supported Only For Legacy Projects)
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.
DNA
Nextera gDNA
“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
Frequently Asked Questions
Please refer to frequently asked questions section for FAQ regarding library prep options.
