Sequencing
From Genome Technology Core (GTC) wiki - Sequencing and Microarray
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GTC has two Illumina Genome Analyzers 2.0. Each sequencer can process up to 16 samples per week, with a data yield of 20-30 million reads per sample. Read lengths are 36 to >100 bases in single or paired-end formats.
Templates are “sequenced” by incorporating fluorescently labeled nucleotides with Illumina's proprietary “Sequencing-By-Synthesis” technology. A detailed description of this process can be found here: http://illumina.com/pages.ilmn?ID=203
Frequently used applications of Illumina sequencing include:
- ChIP Seq
- Small RNA Sequencing
- Digital gene expression
- Whole Transcriptome Analysis
- Genome sequencing and resequencing
- Paired End Sequencing
- Genotyping: Protocols are being developed for detection of SNPs, chromosomal rearrangements and other genotyping applications.
Protocols
Protocols for all of the supported technologies can be found on the Illumina website, or by contacting Illumina at (800) 809-4566. http://illumina.com/pagesnrn.ilmn?ID=70#252
