Difference between revisions of "Sequencing"

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Templates are “sequenced” by incorporating fluorescently labeled nucleotides with Illumina's proprietary “Sequencing-By-Synthesis” technology. A detailed description of this process can be found here: http://illumina.com/pages.ilmn?ID=203
 
Templates are “sequenced” by incorporating fluorescently labeled nucleotides with Illumina's proprietary “Sequencing-By-Synthesis” technology. A detailed description of this process can be found here: http://illumina.com/pages.ilmn?ID=203
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Applications:
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= ChIP-Seq =
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ChIP-Sequencing (or ChIP-Seq) combines chromatin immunoprecipitation (ChIP) with DNA sequencing allowing researchers to identify the binding sites of DNA-associated proteins. a
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Please see below for further details about the service.
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== Sample Submission Guidelines ==
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Users are required to prepare their own sequencing libraries. Samples should be gel-purified and provided UNDILUTED in EB.
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Users should submit 5uL of undiluted sample. We will determine the concentration by RT-PCR and make the appropriate dilutions. Excess sample will be retained in our freezers for potential future re-run. Users are also strongly encouraged to retain a portion of their samples in case of emergency.
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GTC will provide sequencing primers for libraries prepared with standard Illumina adapters. If custom sequencing primers are required they must be provided at the time of sample submission.
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== Data ==
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Images acquired from the solexa sequencer are processed through the bundled solexa inage extraction pipeline to get the sequence and quality score for each base. The data is aligned to a reference genome, if requested, using an interative ELAND algorithm. An in-depth QC report is included in the package. Sample QC report is here.
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Revision as of 13:40, 20 August 2010

Sequencing Service
Dna seq.jpg

GTC has two Illumina Genome Analyzers 2.0. Each sequencer can process up to 16 samples per week, with a data yield of 20-30 million reads per sample. Read lengths are 36 to >100 bases in single or paired-end formats.

Templates are “sequenced” by incorporating fluorescently labeled nucleotides with Illumina's proprietary “Sequencing-By-Synthesis” technology. A detailed description of this process can be found here: http://illumina.com/pages.ilmn?ID=203

Applications:

ChIP-Seq

ChIP-Sequencing (or ChIP-Seq) combines chromatin immunoprecipitation (ChIP) with DNA sequencing allowing researchers to identify the binding sites of DNA-associated proteins. a

Please see below for further details about the service.

Sample Submission Guidelines

Users are required to prepare their own sequencing libraries. Samples should be gel-purified and provided UNDILUTED in EB.

Users should submit 5uL of undiluted sample. We will determine the concentration by RT-PCR and make the appropriate dilutions. Excess sample will be retained in our freezers for potential future re-run. Users are also strongly encouraged to retain a portion of their samples in case of emergency.

GTC will provide sequencing primers for libraries prepared with standard Illumina adapters. If custom sequencing primers are required they must be provided at the time of sample submission.

Data

Images acquired from the solexa sequencer are processed through the bundled solexa inage extraction pipeline to get the sequence and quality score for each base. The data is aligned to a reference genome, if requested, using an interative ELAND algorithm. An in-depth QC report is included in the package. Sample QC report is here.

test


Frequently used applications of Illumina sequencing include:


Protocols

Protocols for all of the supported technologies can be found on the Illumina website, or by contacting Illumina at (800) 809-4566. http://illumina.com/pagesnrn.ilmn?ID=70#252

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