Difference between revisions of "Rnaextraction"
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Latest revision as of 17:05, 22 March 2023
RNA Extraction
We are pleased to offer two methods of RNA extraction. Both methods retain total RNA, including small RNAs.
Qiagen miRNeasy
For most low- to medium-throughput experiments, we recommend the column-based Qiagen miRNeasy.
This kit purifies RNA from up to 5 mg tissue, or cells (up to 1 x 106 cells).
Samples should be provided to the Genome Core frozen in exactly 700 ul of Qiazol (please reach out to us, we can supply this), or Trizol. Please do not use >700 ul of Qiazol.
MagMAX mirVana
For high throughput projects, we recommend the 96-well, plate-based MagMAX mirVana Total RNA Isolation Kit from Thermo Fisher.
https://www.thermofisher.com/order/catalog/product/A27828
This kit purifies RNA from 10-30 mg of tissue or 1 × 106 cells.
Samples should be provided to the Genome Core frozen in the MagMax Processing Plate in MagMax Lysis Binding Mix (please reach out to us, we can supply these), according to your project’s unique specifications. We will work with you to make sure that you have all of the information and supplies.
