RT PCR

The GTC features the 7900HT Fast Real-Time PCR system from Applied Biosystems for quantitative PCR. This highly flexible system supports both 96 and 384 well plate formats as well as the TLDA (TaqMan Low Density Array) card format.

Information about the 7900HT
Information about the TLDA system can be found here

Access and Availability

Access to the 7900HT is available in 2 hour time blocks 24 hours a day, 7 days a week. Advance sign-up is required. It is recommended that you sign up at least 48 hours in advance to ensure availability.

New users should contact Jeong-Ah Kwon [jkwon at wi dot mit dot edu] to schedule an orientation session. Current users who would like to learn how to change between 96 and 384 well plate blocks should also contact Jeong-Ah for training. Please do not attempt to change the blocks unless you have been trained as improper handling can damage the blocks. Jeong-Ah is available during normal business hours for training and consultation.

Due to high demand, access to this equipment is limited to Whitehead Institute users only.

Sign-up policy

• ALL Reserved Slots/Blocks will be charged (unless deleted from the system).
• Reserved Slots/Blocks cannot be deleted AFTER the timeslot begins.
• Overbooking will not be tolerated. If you are identified as some one reserving a lot more slots than you need and then deleting them, you will be penalized.
• Please ensure you complete your run within your alloted timeslots/blocks.

• Logging Reagent usage is mandatory for everyone using the machines. (It helps us send you reminders if you forget to log the reagents)
• If no GTC reagents are used, please log the number of plates and ulmix as 0.
• You will get multiple email reminders to log your reagent usage, if reagent usage is not logged by the 10th of the following Month. If you ignore those emails, we will bill for the maximum potential usage.

The following consumables and reagents are available from the GTC for a fee:

• Optical plates (96-well fast, 384-well)
• Optical Adhesive Films
• TaqMan Universal Master Mix
• SYBR Green Master Mix

Please sign-up for the a time slot to use the machine online. Click Here For Details.

Please note that if you do not delete any scheduled slots for yourself that you are not going to use BEFORE the time slot starts, you would still be billed for them.

Analysis

The analysis software SDS v2.3 is installed on our analysis computer located in room 352. An installation disc is also available if you wish to work on your own computer (Windows only).

Pricing

Item	Unit	Price Per Unit	Notes
SYBR green / Taq Man Master mix	uL	6.5 cents	-
Optical Plate	Plate	$7.00	-
Equipment Time (2 hour time block)	Plate	$30.00	Sign-up Required