Difference between revisions of "Pricing"

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= Illumina Sequencing =
 
= Illumina Sequencing =
 
 
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There are two options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The new HiSeq typically yields 70-100 million reads per lane. Prices include sample quality control for one samples and basic data processing including base calls, quality scores and alignment. Sequencing pricing based on 7 samples per flow cell plus a control lane. 
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There are three options for Illumina sequencing. NovaSeq and MiSeq.
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{{PricingHeader}}
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{{PricingRow|GAII - 36 bp Single Read|Lane|$860|10-30 million reads}}
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Prices include basic data processing including base calls, quality scores and alignment (if requested).  
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{{PricingRow|GAII - 36x36 Paired End|Lane|$1,500|10-30 million paired-end reads}}
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{{PricingRow|GAII - Add'l 36 bases|Lane|$300|Add to above base prices for longer reads}}
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Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
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{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,270|50-100 million reads}}
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{{PricingRow|HiSeq - 40x40 Paired End|Lane|$2,230|50-100 million paired end reads}}
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=== NovaSeq (Single lane submissions accepted for standard read lengths) ===
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{{PricingRow|HiSeq - Add'l 40 bases|Lane|$450|Add to above base prices for longer reads}}
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{{PricingRow|Multiplex Barcode Read|FlowCell|$300|GA or HiSeq, split by up to 7 barcoded lanes}}
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{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
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{{PricingRow|Library prep|Sample|$185|SPRIworks library prep, includes all reagents}}
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|-
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{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
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| Item || Read Length || Unit || Price Per Unit || Notes
 +
|-  
 +
|rowspan="4"|NovaSeqSP
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="4"| 1 lane
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|$1,850
 +
|rowspan="4"|~650–800 million reads per flowcell <br>~325-400 million reads per lane <br>(x2 for Paired-End)
 +
|-  
 +
| 200 Single End or 100x100 Paired-End
 +
|$2,245
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$2,620
 +
|-
 +
| 500 Single End or 250x250 Paired-End
 +
|$3,510
 +
|-
 +
|rowspan="3"|NovaSeqS1
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$2,920
 +
|rowspan="3"|~1.3–1.6 billion reads per flowcell <br> ~650–800 million reads per lane <br> (x2 for Paired-End)
 +
|-  
 +
| 200 Single End or 100x100 Paired-End
 +
|$3,730
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$4,065
 +
|-
 +
|rowspan="3"|NovaSeqS2
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| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$5,200
 +
|rowspan="3"|~3.3–4.1 billion reads per flowcell <br> ~1.65-2.05 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$6,480
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$6,750
 +
|-
 +
|rowspan="2"|NovaSeqS4
 +
| 50 Single End
 +
|rowspan="2"|1 lane
 +
|$3,780
 +
|rowspan="2"|~8-10 billion reads per flowcell <br> ~2-2.5 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$5,400
 +
|-
 +
|}
  
  
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<br>
 
 
<br>
 
<br>
  
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= Microarray Services =
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=== MiSeq ===
 +
{{PricingHeader}}
 +
{{PricingRow|MiSeq v2 70 Single End or 30x30 Paired End|Flowcell|$1,320| 10 million reads}}
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{{PricingRow|MiSeq v3 150 Single End or 75x75 Paired End|Flowcell|$1,430| 20 million reads}}
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{{PricingRow|MiSeq v2 300 Single End or 150x150 Paired End|Flowcell|$1,595| 10 million reads}}
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{{PricingRow|MiSeq v2 500 Single End or 250x250 Paired End|Flowcell|$1,760| 10 million reads}}
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{{PricingLastRow|MiSeq v3 600 Single End or 300x300 Paired End|Flowcell|$2,200| 20 million reads}}
  
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Prices for sample processing do not include cost of arrays
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<br>
  
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== Affymetrix Array Processing==
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= RNA Library Prep =
 +
=== Standard (Manual) ===
 
{{PricingHeader}}
 
{{PricingHeader}}
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{{PricingRow|Affymetrix Gene 1.0 ST |Array|~$175|Array only}}
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{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12|Sample|$200|}}
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{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
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{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+|Sample|$150|}}
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{{PricingRow|Standard Expression Processing|Sample|~$500|>5ug total RNA}}
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{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 1-12|Sample|$250|}}
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{{PricingRow|Small Sample Expression Processing|Sample|~$650|10-100ng total RNA}}
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{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 13+|Sample|$200|}}
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{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}
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{{PricingRow|Agilent SureSelect All Exon|Sample|$650|}}
 +
{{PricingLastRow|NEBNext Small RNA|Sample|$200|}}
  
 
<br>
 
<br>
  
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== Agilent Array Processing==
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=== Highthroughput (96 well Plate Based) ===
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{{PricingHeader}}
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{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
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{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
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{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
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|-
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{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
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| Item || Unit || Price Per Unit || Notes
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{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}
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|-  
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|rowspan="3"|HT mRNA library prep (IDT xGen RNA)
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| 48 Samples
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|$3600
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|rowspan="3" width=50%| Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.
 +
Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.
 +
 
 +
Libraries are pooled by FA quantification, expect variation in read balancing. 
 +
|-
 +
| 49-72 samples
 +
|$5400
 +
|-
 +
| 96 samples
 +
|$7200
 +
|-
 +
|}
  
 
<br>
 
<br>
  
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= nCounter Analysis System (NanoString) =
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= Single Cell (10X and others) =
 
{{PricingHeader}}
 
{{PricingHeader}}
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{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
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{{PricingRow|10X Single Cell 3’ GEX|sample|$2560|}}
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{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
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{{PricingRow|10X Single Cell 5’ GEX|sample|$2560|}}
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{{PricingLastRow|Labor|Cartridge|$105.00|-}}
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{{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}}
 +
{{PricingRow|10X ADT/HTO/CellPlex Add-on|sample|$140|}}
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{{PricingRow|10X Multiome Assay (ATAC + 3')|sample|$4200|}}
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{{PricingRow|10X Flex (Fixed Cell)|sample|$2800|Human and Mouse Only}}
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{{PricingRow|Plexwell Rapid Single Cell - one 96 well plate|96 well plate|$5060|1 cell per well}}
 +
{{PricingLastRow|Plexwell Rapid Single Cell - 1 of 4 96 well plates|96 well plate|$3800|1 cell per well}}
  
 
<br>
 
<br>
−
= Bioanalyzer - RNA, DNA and Protein analysis =
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 +
= DNA Library Prep =
 
{{PricingHeader}}
 
{{PricingHeader}}
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{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}
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{{PricingRow|Swift ChIP|sample|$200|Optimized for low input ChIP}}
 +
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
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{{PricingLastRow|Illumina DNA Prep (Genomic)|sample|$200|Genomic DNA >100ng}}
  
 
<br>
 
<br>
  
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= Quantitative PCR - ABI Prism 7900 =
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= RNA Isolation =
 
{{PricingHeader}}
 
{{PricingHeader}}
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{{PricingRow|SYBR green / Taq Man Master mix|uL|6.5 cents|-}}
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{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation]|sample|$100|}}
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{{PricingRow|Optical Plate|Plate|$7.00|-}}
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{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - MagMax mirVana RNA Isolation]|sample|$30|}}
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{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}
 
  
 
<br>
 
<br>
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= Highthroughput Screening - Cellomics =
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 +
= Quality Control =
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Equipment Time (Internal)|Hourly|$25.00|Sign-up Required}}
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{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
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{{PricingLastRow|Equipment Time (External)|Hourly|$75.00|Sign-up Required}}
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{{PricingRow|qPCR|Sample|$25|Quantification}}
 +
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
  
 
<br>
 
<br>
 +
 +
= Bioanalyzer - RNA, DNA and Protein analysis =
 +
{{PricingHeader}}
 +
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}
 +
 
<br>
 
<br>
−
= Other Services =
 
  
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== Monthly Access Program ==
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= Quantitative PCR - QuantStudio 6 =
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Regular users are encouraged to subscribe to our monthly access program that provides unlimited use of general equipment including the nanodrop spectrophotometer, hybridization chambers and ovens, pipettes and some common supplies. The fee is $250/month and covers the entire lab.
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{{PricingHeader}}
 +
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
 +
{{PricingRow|Optical Plate|Plate|$7|-}}
 +
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30|Sign-up Required}}
 +
 
 +
<br>

Revision as of 12:31, 18 July 2023

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are three options for Illumina sequencing. NovaSeq and MiSeq.

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

NovaSeq (Single lane submissions accepted for standard read lengths)

Item Read Length Unit Price Per Unit Notes
NovaSeqSP 100 Single End or 50x50 Paired-End 1 lane $1,850 ~650–800 million reads per flowcell
~325-400 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $2,245
300 Single End or 150x150 Paired-End $2,620
500 Single End or 250x250 Paired-End $3,510
NovaSeqS1 100 Single End or 50x50 Paired-End 1 lane $2,920 ~1.3–1.6 billion reads per flowcell
~650–800 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $3,730
300 Single End or 150x150 Paired-End $4,065
NovaSeqS2 100 Single End or 50x50 Paired-End 1 lane $5,200 ~3.3–4.1 billion reads per flowcell
~1.65-2.05 billion reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $6,480
300 Single End or 150x150 Paired-End $6,750
NovaSeqS4 50 Single End 1 lane $3,780 ~8-10 billion reads per flowcell
~2-2.5 billion reads per lane
(x2 for Paired-End)
300 Single End or 150x150 Paired-End $5,400



MiSeq

Item Unit Price Per Unit Notes
MiSeq v2 70 Single End or 30x30 Paired End Flowcell $1,320 10 million reads
MiSeq v3 150 Single End or 75x75 Paired End Flowcell $1,430 20 million reads
MiSeq v2 300 Single End or 150x150 Paired End Flowcell $1,595 10 million reads
MiSeq v2 500 Single End or 250x250 Paired End Flowcell $1,760 10 million reads
MiSeq v3 600 Single End or 300x300 Paired End Flowcell $2,200 20 million reads


RNA Library Prep

Standard (Manual)

Item Unit Price Per Unit Notes
IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12 Sample $200
IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+ Sample $150
KAPA Hyper Prep Ribo Zero, samples 1-12 Sample $250
KAPA Hyper Prep Ribo Zero, samples 13+ Sample $200
Agilent SureSelect All Exon Sample $650
NEBNext Small RNA Sample $200


Highthroughput (96 well Plate Based)

Item Unit Price Per Unit Notes
HT mRNA library prep (IDT xGen RNA) 48 Samples $3600 Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.

Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.

Libraries are pooled by FA quantification, expect variation in read balancing. 

49-72 samples $5400
96 samples $7200


Single Cell (10X and others)

Item Unit Price Per Unit Notes
10X Single Cell 3’ GEX sample $2560
10X Single Cell 5’ GEX sample $2560
10X VDJ Add-on sample $230 Can be added onto the 10X 5' GEX Only
10X ADT/HTO/CellPlex Add-on sample $140
10X Multiome Assay (ATAC + 3') sample $4200
10X Flex (Fixed Cell) sample $2800 Human and Mouse Only
Plexwell Rapid Single Cell - one 96 well plate 96 well plate $5060 1 cell per well
Plexwell Rapid Single Cell - 1 of 4 96 well plates 96 well plate $3800 1 cell per well


DNA Library Prep

Item Unit Price Per Unit Notes
Swift ChIP sample $200 Optimized for low input ChIP
Nextera gDNA (Genomic, ATAC-Seq) sample $200 "Tagmentation" prep from intact gDNA (50ng)
Illumina DNA Prep (Genomic) sample $200 Genomic DNA >100ng


RNA Isolation

Item Unit Price Per Unit Notes
RNA-miRNeasy Total RNA Isolation sample $100
RNA - MagMax mirVana RNA Isolation sample $30


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7 -
Equipment Time (2 hour time block) Plate $30 Sign-up Required


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