Difference between revisions of "Pricing"

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{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2,100|Sorting and library prep for up to 10,000 cells}}
 
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2,100|Sorting and library prep for up to 10,000 cells}}
 
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 5’ Solution Library prep (RNA-Seq)]|Sample|$2,250|Sorting and library prep for up to 10,000 cells}}
 
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/SingleCell 10X Single Cell 5’ Solution Library prep (RNA-Seq)]|Sample|$2,250|Sorting and library prep for up to 10,000 cells}}
{{PricingLastRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2,500|}}
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{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2,500|}}
  
 
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Revision as of 10:07, 2 April 2021

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are three options for Illumina sequencing. NovaSeq, HiSeq "Standard" and HiSeq "Rapid".

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

NovaSeq

Item Read Length Unit Price Per Unit Notes
NovaSeqSP 100 Single End or 50x50 Paired-End Flow Cell (2 Lanes) $3,365 ~650–800 million reads per flowcell
~325-400 reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $4,150
300 Single End or 150x150 Paired-End $5,200
500 Single End or 250x250 Paired-End $6,400
NovaSeqS1 100 Single End or 50x50 Paired-End Flow Cell (2 Lanes) $5,300 ~1.3–1.6 billion reads per flowcell
~650–800 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $6,900
300 Single End or 150x150 Paired-End $7,750
NovaSeqS2 100 Single End or 50x50 Paired-End Flow Cell (2 Lanes) $9,500 ~3.3–4.1 billion reads per flowcell
~1.65-2.05 billion reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $11,500
300 Single End or 150x150 Paired-End $12,600
NovaSeqS4 35 Single End Flow Cell (4 Lanes) $14,000 ~8-10 billion reads per flowcell
~2-2.5 billion reads per lane
(x2 for Paired-End)
300 Single End or 150x150 Paired-End $20,000
Add XP protocol - SP,S1,S2 Any Read Length Flow Cell $400 Allows different library pools in each lane
Add XP protocol - S4 Any Read Length Flow Cell $1,200 Allows different library pools in each lane



HiSeq (Std a.k.a. "High Output" mode) -- At least 150-200 million reads per lane

Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

Item Unit Price Per Unit Notes
HiSeq - 50 bp Single Read Lane $1,200
HiSeq - 28x40 10X Lane $1,500 ONLY FOR 10X LIBRARIES
HiSeq - 60x60 Paired End Lane $1,750
HiSeq - 100x100 Paired End Lane $2,250
HiSeq - Additional 70 bases Lane $400 Add to above base prices for longer reads


HiSeq (Rapid) -- At least 100 to 150 million reads per lane (Two lanes per Flow Cell)

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.

Item Unit Price Per Unit Notes
HiSeqRapid - 50 bp Single Read full flowcell (2 lanes) $2,300
HiSeqRapid - 28x40 10X full flowcell (2 lanes) $2,800 ONLY FOR 10X LIBRARIES
HiSeqRapid - 60x60 Paired End full flowcell (2 lanes) $3,400
HiSeqRapid - 100x100 Paired End full flowcell (2 lanes) $4,350
HiSeqRapid - 250x250 Paired End full flowcell (2 lanes) $7,200
HiSeqRapid - Add DUAL mode full flowcell (2 lanes) $460 ONLY required if running different samples in each lane
HiSeqRapid - Additional 70 bases full flowcell (2 lanes) $560 Add to above base prices for longer reads up to 250x250


Library Prep

Item Unit Price Per Unit Notes
Swift ChIP DNA (ChIP-Seq) Sample $200 Optimized for low input ChIP
TruSeq Nano gDNA (Genomic) Sample $200 Genomic DNA >100ng
Nextera gDNA (Genomic, ATAC-Seq) Sample $200 "Tagmentation" prep from intact gDNA (50ng)
KAPA Hyper Prep - Stranded (RNA-Seq) Sample $200
KAPA Ribo Erase (RNA-Seq) Sample $250
Clontech UltraLow RNA (RNA-Seq) Sample $300 For low input RNA down to single cell
Clontech Stranded Total RNA Pico (RNA-Seq) Sample $250 -
NEBNext Small RNA (microRNA) Sample $200 -
10X Single Cell 3’ Solution Library prep (RNA-Seq) Sample $2,100 Sorting and library prep for up to 10,000 cells
10X Single Cell 5’ Solution Library prep (RNA-Seq) Sample $2,250 Sorting and library prep for up to 10,000 cells
SMART-seq v2 Library prep (RNA-Seq) 96-well Plate $2,500
Click here for more info about Library Prep options


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run



nCounter Analysis System (NanoString)

Item Unit Price Per Unit Notes
Reagents Cartridge ---- Reagents and Codesets Purchase From Nanostring
Equipment Use Cartridge $250 12 samples per cartridge
Labor-Hyb Cartridge $150 -
Labor-miRNA Sample $25 Addon for miRNA protocol only


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7 -
Equipment Time (2 hour time block) Plate $30 Sign-up Required