Difference between revisions of "Pricing"

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There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".
 
There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".
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In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 150-200 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
 
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In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.
 
  
 
Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.  
 
Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.  
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Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
 
Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
  
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=== HiSeq (Std) -- atleast 150-200 million reads per lane ===
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=== HiSeq (Std a.k.a. "High Output" mode) -- atleast 150-200 million reads per lane ===
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===== Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run. =====
 
{{PricingHeader}}
 
{{PricingHeader}}
 
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,140|}}
 
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,140|}}
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=== HiSeq (Rapid) -- atleast 100 to 150 million reads per lane ===
 
=== HiSeq (Rapid) -- atleast 100 to 150 million reads per lane ===
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===== Rapid runs finish faster than HiSeq (Std) - 100x100 takes 2-3 days on rapid mode vs 7 days on std mode plus wait time to fill out all 8 HiSeq (Std) lanes =====
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===== In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250. Rapid runs also complete more quickly, for example - a 100x100 run on rapid completes in 2-3 days vs can take up to 7 days in standard mode. =====
 
{{PricingHeader}}
 
{{PricingHeader}}
 
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,680|}}
 
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$1,680|}}

Revision as of 11:55, 20 September 2017

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

HiSeq (Std a.k.a. "High Output" mode) -- atleast 150-200 million reads per lane

Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
Item Unit Price Per Unit Notes
HiSeq - 40 bp Single Read Lane $1,140
HiSeq - 26x40 10X Lane $1,300 ONLY FOR 10X LIBRARIES
HiSeq - 40x40 Paired End Lane $1,710
HiSeq - 75x75 Paired End Lane $2,260
HiSeq - 100x100 Paired End Lane $2,700
HiSeq - Additional 40 bases Lane $330 Add to above base prices for longer reads


HiSeq (Rapid) -- atleast 100 to 150 million reads per lane

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250. Rapid runs also complete more quickly, for example - a 100x100 run on rapid completes in 2-3 days vs can take up to 7 days in standard mode.
Item Unit Price Per Unit Notes
HiSeqRapid - 50 bp Single Read full flowcell (2 lanes) $1,680
HiSeqRapid - 40 bp Single Read 1 lane $1,140
HiSeqRapid - 26x40 10X full flowcell (2 lanes) $2,400 ONLY FOR 10X LIBRARIES
HiSeqRapid - 50x50 Paired End full flowcell (2 lanes) $2,500
HiSeqRapid - 50x50 Paired End 1 lane $1,500
HiSeqRapid - 100x100 Paired End full flowcell (2 lanes) $3,250
HiSeqRapid - 100x100 Paired End 1 lane $1,850
HiSeqRapid - 250x250 Paired End full flowcell (2 lanes) $5,550
HiSeqRapid - Add DUAL mode full flowcell (2 lanes) $450 ONLY required if running different samples in each lane
HiSeqRapid - Additional 50 bases full flowcell (2 lanes) $500 Add to above base prices for longer reads up to 250x250


Library Prep

Item Unit Price Per Unit Notes
Swift ChIP DNA (ChIP-Seq) Sample $200 Optimized for low input ChIP
TruSeq Nano gDNA (Genomic) Sample $200 Genomic DNA >100ng
Nextera gDNA (Genomic, ATAC-Seq) Sample $200 "Tagmentation" prep from intact gDNA (50ng)
TruSeq Stranded mRNA (RNA-Seq) Sample $200 Basic RNA Expression Analysis
TruSeq Stranded RiboZero (RNA-Seq) Sample $250 Replaces polyA selection with a ribozomal RNA depletion
Clontech UltraLow RNA (RNA-Seq) Sample $300 For low input RNA down to single cell
Clontech Stranded Total RNA Pico (RNA-Seq) Sample $250 -
NEBNext Small RNA (microRNA) Sample $200 -
10X Single Cell 3’ Solution Library prep (RNA-Seq) Sample $1800 Sorting and library prep for up to 10,000 cells
SMART-seq v2 Library prep (RNA-Seq) 96-well Plate $2400


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Microarray Services

Prices for sample processing do not include cost of arrays

Affymetrix Array Processing

Item Unit Price Per Unit Notes
Full Service Expression Analysis - 3'IVT or WT Sample $325 Not including cost of Array
Contact GTC or Affymetrix for array prices Array $150-$400 Array only



Agilent Array Processing

Item Unit Price Per Unit Notes
Full Service Expression Analysis (Single Channel) Sample $325 Not including cost of array
Full Service Expression Analysis (Two Samples - Dual Channel) Two Samples $525 Not including cost of array
Contact GTC or Agilent for Array pricing Array $$



nCounter Analysis System (NanoString)

Item Unit Price Per Unit Notes
Equipment Use Cartridge $250 12 samples per cartridge
Reagents Cartridge $280.50* *Does NOT include CodeSet
Labor-Hyb Cartridge $150.00 -
Labor-miRNA Sample $25.00 -


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7.00 -
Equipment Time (2 hour time block) Plate $30.00 Sign-up Required


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