Difference between revisions of "Pricing"

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= Illumina Sequencing =
 
= Illumina Sequencing =
 
 
−
There are three options for Illumina sequencing. NovaSeq, HiSeq "Standard" and HiSeq "Rapid".
+
There are three options for Illumina sequencing. NovaSeq and MiSeq.
  
 
Prices include basic data processing including base calls, quality scores and alignment (if requested).  
 
Prices include basic data processing including base calls, quality scores and alignment (if requested).  
Line 10: Line 10:
 
Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
 
Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
  
−
=== NovaSeq ===
+
=== NovaSeq (Single lane submissions accepted for standard read lengths) ===
  
 
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
Line 18: Line 18:
 
|rowspan="4"|NovaSeqSP
 
|rowspan="4"|NovaSeqSP
 
| 100 Single End or 50x50 Paired-End
 
| 100 Single End or 50x50 Paired-End
−
|rowspan="4"|Flow Cell (2 Lanes)
+
|rowspan="4"| 1 lane
−
|$3,365
+
|$1,850
−
|rowspan="4"|~650–800 million reads per flowcell <br>~325-400 reads per lane <br>(x2 for Paired-End)  
+
|rowspan="4"|~650–800 million reads per flowcell <br>~325-400 million reads per lane <br>(x2 for Paired-End)  
 
|-  
 
|-  
 
| 200 Single End or 100x100 Paired-End
 
| 200 Single End or 100x100 Paired-End
−
|$4,150
+
|$2,245
 
|-  
 
|-  
 
| 300 Single End or 150x150 Paired-End
 
| 300 Single End or 150x150 Paired-End
−
|$5,200
+
|$2,620
 
|-
 
|-
 
| 500 Single End or 250x250 Paired-End
 
| 500 Single End or 250x250 Paired-End
−
|$6,400
+
|$3,510
 
|-
 
|-
 
|rowspan="3"|NovaSeqS1
 
|rowspan="3"|NovaSeqS1
 
| 100 Single End or 50x50 Paired-End
 
| 100 Single End or 50x50 Paired-End
−
|rowspan="3"|Flow Cell (2 Lanes)
+
|rowspan="3"|1 lane
−
|$5,300
+
|$2,920
 
|rowspan="3"|~1.3–1.6 billion reads per flowcell <br> ~650–800 million reads per lane <br> (x2 for Paired-End)
 
|rowspan="3"|~1.3–1.6 billion reads per flowcell <br> ~650–800 million reads per lane <br> (x2 for Paired-End)
 
|-  
 
|-  
 
| 200 Single End or 100x100 Paired-End
 
| 200 Single End or 100x100 Paired-End
−
|$6,900
+
|$3,730
 
|-
 
|-
 
| 300 Single End or 150x150 Paired-End
 
| 300 Single End or 150x150 Paired-End
−
|$7,750
+
|$4,065
 
|-
 
|-
 
|rowspan="3"|NovaSeqS2
 
|rowspan="3"|NovaSeqS2
 
| 100 Single End or 50x50 Paired-End
 
| 100 Single End or 50x50 Paired-End
−
|rowspan="3"|Flow Cell (2 Lanes)
+
|rowspan="3"|1 lane
−
|$9,500
+
|$5,200
 
|rowspan="3"|~3.3–4.1 billion reads per flowcell <br> ~1.65-2.05 billion reads per lane <br> (x2 for Paired-End)
 
|rowspan="3"|~3.3–4.1 billion reads per flowcell <br> ~1.65-2.05 billion reads per lane <br> (x2 for Paired-End)
 
|-  
 
|-  
 
| 200 Single End or 100x100 Paired-End
 
| 200 Single End or 100x100 Paired-End
−
|$11,500
+
|$6,480
 
|-
 
|-
 
| 300 Single End or 150x150 Paired-End
 
| 300 Single End or 150x150 Paired-End
−
|$12,600
+
|$6,750
 
|-
 
|-
 
|rowspan="2"|NovaSeqS4  
 
|rowspan="2"|NovaSeqS4  
−
| 35 Single End
+
| 50 Single End
−
|rowspan="2"|Flow Cell (4 Lanes)
+
|rowspan="2"|1 lane
−
|$14,000
+
|$3,780
 
|rowspan="2"|~8-10 billion reads per flowcell <br> ~2-2.5 billion reads per lane <br> (x2 for Paired-End)
 
|rowspan="2"|~8-10 billion reads per flowcell <br> ~2-2.5 billion reads per lane <br> (x2 for Paired-End)
 
|-
 
|-
 
| 300 Single End or 150x150 Paired-End
 
| 300 Single End or 150x150 Paired-End
−
|$20,000
+
|$5,400
 +
|-
 +
|}
 +
 
 +
 
 +
<br>
 +
 
 +
=== MiSeq ===
 +
{{PricingHeader}}
 +
{{PricingRow|MiSeq v2 70 Single End or 30x30 Paired End|Flowcell|$1,320| 10 million reads}}
 +
{{PricingRow|MiSeq v3 150 Single End or 75x75 Paired End|Flowcell|$1,430| 20 million reads}}
 +
{{PricingRow|MiSeq v2 300 Single End or 150x150 Paired End|Flowcell|$1,595| 10 million reads}}
 +
{{PricingRow|MiSeq v2 500 Single End or 250x250 Paired End|Flowcell|$1,760| 10 million reads}}
 +
{{PricingLastRow|MiSeq v3 600 Single End or 300x300 Paired End|Flowcell|$2,200| 20 million reads}}
 +
 
 +
<br>
 +
 
 +
= RNA Library Prep =
 +
=== Standard (Manual) ===
 +
{{PricingHeader}}
 +
{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12|Sample|$200|}}
 +
{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+|Sample|$150|}}
 +
{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 1-12|Sample|$250|}}
 +
{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 13+|Sample|$200|}}
 +
{{PricingRow|Agilent SureSelect All Exon|Sample|$650|}}
 +
{{PricingLastRow|NEBNext Small RNA|Sample|$200|}}
 +
 
 +
<br>
 +
 
 +
=== Highthroughput (96 well Plate Based) ===
 +
 
 +
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 +
|-
 +
| Item || Unit || Price Per Unit || Notes
 
|-  
 
|-  
−
|Add XP protocol - SP,S1,S2
+
|rowspan="3"|HT mRNA library prep (IDT xGen RNA)
−
|Any Read Length
+
| 48 Samples
−
|Flow Cell
+
|$3600
−
|$400
+
|rowspan="3" width=50%| Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.
−
|Allows different library pools in each lane
+
Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.
 +
 
 +
Libraries are pooled by FA quantification, expect variation in read balancing. 
 +
|-
 +
| 49-72 samples
 +
|$5400
 
|-
 
|-
−
|Add XP protocol - S4
+
| 96 samples
−
|Any Read Length
+
|$7200
−
|Flow Cell
 
−
|$1,200
 
−
|Allows different library pools in each lane
 
 
|-
 
|-
 
|}
 
|}
−
 
  
 
<br>
 
<br>
  
−
=== HiSeq (Std a.k.a. "High Output" mode) -- At least 150-200 million reads per lane ===
+
= Single Cell (10X and others) =
−
Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
 
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|HiSeq - 50 bp Single Read|Lane|$1,200|}}
+
{{PricingRow|10X Single Cell 3’ GEX|sample|$2560|}}
−
{{PricingRow|HiSeq - 28x40 10X|Lane|$1,500|ONLY FOR 10X LIBRARIES}}
+
{{PricingRow|10X Single Cell 5’ GEX|sample|$2560|}}
−
{{PricingRow|HiSeq - 60x60 Paired End|Lane|$1,750|}}
+
{{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}}
−
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,250|}}
+
{{PricingRow|10X ADT/HTO/CellPlex Add-on|sample|$140|}}
−
{{PricingRow|HiSeq - Additional 70 bases|Lane|$400|Add to above base prices for longer reads}}
+
{{PricingRow|10X Multiome Assay (ATAC + 3')|sample|$4200|}}
 +
{{PricingRow|10X Flex (Fixed Cell)|sample|$2800|Human and Mouse Only}}
 +
{{PricingRow|Plexwell Rapid Single Cell - one 96 well plate|96 well plate|$5060|1 cell per well}}
 +
{{PricingLastRow|Plexwell Rapid Single Cell - 1 of 4 96 well plates|96 well plate|$3800|1 cell per well}}
  
−
{{PricingLastRow||||}}
 
 
<br>
 
<br>
  
−
=== HiSeq (Rapid) -- At least 100 to 150 million reads per lane (Two lanes per Flow Cell) ===
+
= DNA Library Prep =
−
In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.
 
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|HiSeqRapid - 50 bp Single Read|full flowcell (2 lanes)|$2,300|}}
+
{{PricingRow|Swift ChIP|sample|$200|Optimized for low input ChIP}}
−
{{PricingRow|HiSeqRapid - 28x40 10X|full flowcell (2 lanes)|$2,800|ONLY FOR 10X LIBRARIES}}
+
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
−
{{PricingRow|HiSeqRapid - 60x60 Paired End|full flowcell (2 lanes)|$3,400|}}
+
{{PricingLastRow|Illumina DNA Prep (Genomic)|sample|$200|Genomic DNA >100ng}}
−
{{PricingRow|HiSeqRapid - 100x100 Paired End|full flowcell (2 lanes)|$4,350|}}
 
−
{{PricingRow|HiSeqRapid - 250x250 Paired End|full flowcell (2 lanes)|$7,200|}}
 
−
{{PricingRow| | | | }}
 
−
{{PricingRow|HiSeqRapid - Add DUAL mode|full flowcell (2 lanes)|$460| ONLY required if running different samples in each lane}}
 
−
{{PricingRow| | | | }}
 
−
{{PricingRow|HiSeqRapid - Additional 70 bases|full flowcell (2 lanes)|$560|Add to above base prices for longer reads up to 250x250}}
 
−
{{PricingLastRow||||}}
 
  
 
<br>
 
<br>
  
−
=== Library Prep ===
+
= RNA Isolation =
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Swift ChIP DNA (ChIP-Seq)|Sample|$200|Optimized for low input ChIP}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation]|sample|$100|}}
−
{{PricingRow|TruSeq Nano gDNA (Genomic)|Sample|$200|Genomic DNA >100ng}}
+
{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - MagMax mirVana RNA Isolation]|sample|$30|}}
−
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|Sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
 
−
{{PricingRow|KAPA Hyper Prep - Stranded (RNA-Seq)|Sample|$200|}}
 
−
{{PricingRow|KAPA Ribo Erase (RNA-Seq)|Sample|$250|}}
 
−
{{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}}
 
−
{{PricingRow|Clontech Stranded Total RNA Pico (RNA-Seq)|Sample|$250|-}}
 
−
{{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}}
 
−
{{PricingRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell 10X Single Cell 3’ Solution Library prep (RNA-Seq)]|Sample|$2,000|Sorting and library prep for up to 10,000 cells}}
 
−
{{PricingLastRow|[http://jura.wi.mit.edu/genomecorewiki/index.php/SingleCell SMART-seq v2 Library prep (RNA-Seq)]|96-well Plate|$2,500|}}
 
  
−
{| style="background:white; color:black;" align="right"
 
−
| [[LibraryPrep|Click here for more info about Library Prep options]]
 
−
|}
 
 
<br>
 
<br>
  
−
=== Quality Control ===
+
= Quality Control =
 
{{PricingHeader}}
 
{{PricingHeader}}
 
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
 
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
 
{{PricingRow|qPCR|Sample|$25|Quantification}}
 
{{PricingRow|qPCR|Sample|$25|Quantification}}
 
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 +
 
<br>
 
<br>
  
−
 
−
= nCounter Analysis System (NanoString) =
 
−
{{PricingHeader}}
 
−
{{PricingRow|Reagents|Cartridge|----|Reagents and Codesets Purchase From Nanostring}}
 
−
{{PricingRow|Equipment Use|Cartridge|$250|12 samples per cartridge}}
 
−
{{PricingRow|Labor-Hyb|Cartridge|$150|-}}
 
−
{{PricingLastRow|Labor-miRNA|Sample|$25|Addon for miRNA protocol only}}
 
−
 
−
<br>
 
 
= Bioanalyzer - RNA, DNA and Protein analysis =
 
= Bioanalyzer - RNA, DNA and Protein analysis =
 
{{PricingHeader}}
 
{{PricingHeader}}

Latest revision as of 12:31, 18 July 2023

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are three options for Illumina sequencing. NovaSeq and MiSeq.

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

NovaSeq (Single lane submissions accepted for standard read lengths)

Item Read Length Unit Price Per Unit Notes
NovaSeqSP 100 Single End or 50x50 Paired-End 1 lane $1,850 ~650–800 million reads per flowcell
~325-400 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $2,245
300 Single End or 150x150 Paired-End $2,620
500 Single End or 250x250 Paired-End $3,510
NovaSeqS1 100 Single End or 50x50 Paired-End 1 lane $2,920 ~1.3–1.6 billion reads per flowcell
~650–800 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $3,730
300 Single End or 150x150 Paired-End $4,065
NovaSeqS2 100 Single End or 50x50 Paired-End 1 lane $5,200 ~3.3–4.1 billion reads per flowcell
~1.65-2.05 billion reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $6,480
300 Single End or 150x150 Paired-End $6,750
NovaSeqS4 50 Single End 1 lane $3,780 ~8-10 billion reads per flowcell
~2-2.5 billion reads per lane
(x2 for Paired-End)
300 Single End or 150x150 Paired-End $5,400



MiSeq

Item Unit Price Per Unit Notes
MiSeq v2 70 Single End or 30x30 Paired End Flowcell $1,320 10 million reads
MiSeq v3 150 Single End or 75x75 Paired End Flowcell $1,430 20 million reads
MiSeq v2 300 Single End or 150x150 Paired End Flowcell $1,595 10 million reads
MiSeq v2 500 Single End or 250x250 Paired End Flowcell $1,760 10 million reads
MiSeq v3 600 Single End or 300x300 Paired End Flowcell $2,200 20 million reads


RNA Library Prep

Standard (Manual)

Item Unit Price Per Unit Notes
IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12 Sample $200
IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+ Sample $150
KAPA Hyper Prep Ribo Zero, samples 1-12 Sample $250
KAPA Hyper Prep Ribo Zero, samples 13+ Sample $200
Agilent SureSelect All Exon Sample $650
NEBNext Small RNA Sample $200


Highthroughput (96 well Plate Based)

Item Unit Price Per Unit Notes
HT mRNA library prep (IDT xGen RNA) 48 Samples $3600 Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.

Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.

Libraries are pooled by FA quantification, expect variation in read balancing. 

49-72 samples $5400
96 samples $7200


Single Cell (10X and others)

Item Unit Price Per Unit Notes
10X Single Cell 3’ GEX sample $2560
10X Single Cell 5’ GEX sample $2560
10X VDJ Add-on sample $230 Can be added onto the 10X 5' GEX Only
10X ADT/HTO/CellPlex Add-on sample $140
10X Multiome Assay (ATAC + 3') sample $4200
10X Flex (Fixed Cell) sample $2800 Human and Mouse Only
Plexwell Rapid Single Cell - one 96 well plate 96 well plate $5060 1 cell per well
Plexwell Rapid Single Cell - 1 of 4 96 well plates 96 well plate $3800 1 cell per well


DNA Library Prep

Item Unit Price Per Unit Notes
Swift ChIP sample $200 Optimized for low input ChIP
Nextera gDNA (Genomic, ATAC-Seq) sample $200 "Tagmentation" prep from intact gDNA (50ng)
Illumina DNA Prep (Genomic) sample $200 Genomic DNA >100ng


RNA Isolation

Item Unit Price Per Unit Notes
RNA-miRNeasy Total RNA Isolation sample $100
RNA - MagMax mirVana RNA Isolation sample $30


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7 -
Equipment Time (2 hour time block) Plate $30 Sign-up Required


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