New
Announcing several new services and technologies available now at the Genome Technology Core.
Contents
HiSeq 2000 - New Technology
The HiSeq2000 from Illumina has the highest data yield of any commercially available sequencer. The latest model in the industry leading Illumina line offers up to 100 million reads per lane. Read lengths over 100 bases are possible in single read or paired-end mode. Data yield is ~2.5x higher than the previous GAII models for only 50% more cost. We will continue to operate our GAII sequencers at a reduced cost for users who don’t need the higher data yields and prefer a less expensive run cost.
TruSeq Multiplex Sequencing - New Technology
Reduce costs even further with sample multiplexing. Illumina’s new TruSeq library prep kits allow multiplexing of up to 12 samples in a single lane. Compatible with both the HiSeq and GAII sequencers these new kits will replace the old prep kits which are being phased out.
SPRI Based Library Prep for Sequencing - New Service
The GTC now offers library prep services for Illumina sequencing. The SPRIworks system from Beckman automates the library prep protocol including the laborious size selection. Not only is this a significant time and labor savings but it can also improve library complexity and increase the percentage of aligned reads. The input is dsDNA (RNA samples need to be reverse transcribed first) and the service includes all adaptors and reagents for a cost of $185. The system is compatible with the TruSeq reagents allowing for preparation of barcoded samples for multiplexing.
NanoString - New(ish) Technology
Last fall the GTC launched a new service with the nCounter system from Nanostring. The nCounter is a “mid-multiplex” technology ideal for measuring dozens to hundreds of targets in dozens up to thousands of samples. Filling the throughput and cost niche between RT-PCR and microarrays the system offers a simple, affordable and customizable option for gene expression analysis and other applications.
Cellomics - New(ish) Technology
The Cellomics ArrayScan High Content Screening platform formerly housed at the MIT Imaging facility has a new home at the GTC. The usage cost is $25/hour and reservations can be made with our online calendar.
New Pricing
A new price list for 2011 has been released. The new list features pricing for all our new services including a reduced price for Illumina sequencing with the GAII machines. The new pricing will be in effect for all services performed after January 1st 2011.
