NanoString
|
ChIP-Sequencing (or ChIP-Seq) combines chromatin immunoprecipitation (ChIP) with DNA sequencing allowing researchers to identify the binding sites of DNA-associated proteins. a
Please see below for further details about the service.
Sample Submission Guidelines
Users are required to prepare their own sequencing libraries. Samples should be gel-purified and provided UNDILUTED in EB.
Users should submit 5uL of undiluted sample. We will determine the concentration by RT-PCR and make the appropriate dilutions. Excess sample will be retained in our freezers for potential future re-run. Users are also strongly encouraged to retain a portion of their samples in case of emergency.
GTC will provide sequencing primers for libraries prepared with standard Illumina adapters. If custom sequencing primers are required they must be provided at the time of sample submission.
Data
Images acquired from the solexa sequencer are processed through the bundled solexa inage extraction pipeline to get the sequence and quality score for each base. The data is aligned to a reference genome, if requested, using an interative ELAND algorithm. An in-depth QC report is included in the package. Sample QC report is here.
test
Turnaround Time
Wait times vary but are generally two weeks or less.
Pricing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Sequencing - 36 bp Single Read | Sample | ~$850 | Cluster, Sequence, Pipeline |
| Sequencing - 36 bp Paired-End Reads | Sample | ~$1,500 | Cluster, Sequence, Pipeline |
| Sample prep - ChIP Seq | Sample | Inquire | - |
| Genome Alignment and other analysis | Sample | Inquire | - |
