Difference between revisions of "NanoString"
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| − | + | The nCounter™ Analysis System measures abundance by tagging with barcodes and counting individual mRNA molecules using a novel digital technology. This system is able to detect 1 copy of mRNA per cell, can evaluate up to 800 genes per reaction, and requires only 10-100 ng of total RNA for analysis. Nanostrings can also be used for miRNA profiling and CNV analysis. | |
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== Sample Submission Guidelines == | == Sample Submission Guidelines == | ||
Revision as of 12:14, 21 September 2010
The nCounter™ Analysis System measures abundance by tagging with barcodes and counting individual mRNA molecules using a novel digital technology. This system is able to detect 1 copy of mRNA per cell, can evaluate up to 800 genes per reaction, and requires only 10-100 ng of total RNA for analysis. Nanostrings can also be used for miRNA profiling and CNV analysis.
Sample Submission Guidelines
Data
How do you normalize the data?
First normalize using the internal system controls to remove any slight assay-to-assay system variation and then normalize using 3-4 validated endogenous controls.
Can I compare the nCounter System with my microarray data?
Yes. The fold change data obtained from an nCounter analysis should correlate with fold change results obtained from microarray analyses.
Can nCounter data be used together with qPCR data?
Yes, both in terms of relative expression levels and fold changes.
How do I analyze my data?
The comma separated value file returned by the nCounter system is easily imported and analyzed in other programs, including Excel.
Is there documentation available about analyzing my data?
Yes, there is a data analysis guidelines document that is available in the Product Literature from Nanostring. You can download it here:
Turnaround Time
Pricing
Please refer to the Pricing Section
