Difference between revisions of "NanoString"
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== Data == | == Data == | ||
| − | + | === How do you normalize the data? === | |
| + | First normalize using the internal system controls to remove any slight assay-to-assay system variation and then normalize using 3-4 validated endogenous controls. | ||
| + | |||
| + | === Can I compare the nCounter System with my microarray data? === | ||
| + | Yes. The fold change data obtained from an nCounter analysis should correlate with fold change results obtained from microarray analyses. | ||
| + | |||
| + | === Can nCounter data be used together with qPCR data? === | ||
| + | Yes, both in terms of relative expression levels and fold changes. | ||
| + | |||
| + | === How do I analyze my data? === | ||
| + | The comma separated value file returned by the nCounter system is easily imported and analyzed in other programs, including Excel. | ||
| + | |||
| + | === Is there documentation available about analyzing my data? === | ||
| + | Yes, there is a data analysis guidelines document that is available in the Product Literature from Nanostring. You can download it here: | ||
| − | |||
== Turnaround Time == | == Turnaround Time == | ||
Revision as of 11:50, 21 September 2010
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ChIP-Sequencing (or ChIP-Seq) combines chromatin immunoprecipitation (ChIP) with DNA sequencing allowing researchers to identify the binding sites of DNA-associated proteins. a
Please see below for further details about the service.
Sample Submission Guidelines
Users are required to prepare their own sequencing libraries. Samples should be gel-purified and provided UNDILUTED in EB.
Users should submit 5uL of undiluted sample. We will determine the concentration by RT-PCR and make the appropriate dilutions. Excess sample will be retained in our freezers for potential future re-run. Users are also strongly encouraged to retain a portion of their samples in case of emergency.
GTC will provide sequencing primers for libraries prepared with standard Illumina adapters. If custom sequencing primers are required they must be provided at the time of sample submission.
Data
How do you normalize the data?
First normalize using the internal system controls to remove any slight assay-to-assay system variation and then normalize using 3-4 validated endogenous controls.
Can I compare the nCounter System with my microarray data?
Yes. The fold change data obtained from an nCounter analysis should correlate with fold change results obtained from microarray analyses.
Can nCounter data be used together with qPCR data?
Yes, both in terms of relative expression levels and fold changes.
How do I analyze my data?
The comma separated value file returned by the nCounter system is easily imported and analyzed in other programs, including Excel.
Is there documentation available about analyzing my data?
Yes, there is a data analysis guidelines document that is available in the Product Literature from Nanostring. You can download it here:
Turnaround Time
Wait times vary but are generally two weeks or less.
Pricing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Sequencing - 36 bp Single Read | Sample | ~$850 | Cluster, Sequence, Pipeline |
| Sequencing - 36 bp Paired-End Reads | Sample | ~$1,500 | Cluster, Sequence, Pipeline |
| Sample prep - ChIP Seq | Sample | Inquire | - |
| Genome Alignment and other analysis | Sample | Inquire | - |
