ChIP on Chip
From Genome Technology Core (GTC) wiki - Sequencing and Microarray
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We offer a ChIP-CHIP Service utilizing the 3 Plaforms.
Key highlights of the service:
Please see below for further details about the service.
Contents
Sample Submission Guidelines
- Customer should submit atleast 5 ug of Total RNA or 2 ug of Samples enriched for miRNA.
- RNA concentration and quality should be quantified.
- Sample submission forms should be submitted along or before the samples are submitted. The form is available here.
mRNA Detection Platform
- Affymetrix.
- Agilent
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Hyb Quality Control
Data Extraction
- Microarray images are scanned for a balanced view and saved in TIFF formats.
- Numerical intensities are extracted for each probes
- The results are organized which includes TIFF image files and intensity data files, which is made available to the customer in a CD.