Pricing

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

Illumina Sequencing

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

HiSeq (Std)

Item	Unit	Price Per Unit	Notes
HiSeq - 40 bp Single Read	Lane	$1,140	avg 120-150 million reads
HiSeq - 40x40 Paired End	Lane	$1,710	avg 120-150 million paired end reads
HiSeq - 75x75 Paired End	Lane	$2,260	avg 120-150 million paired end reads
HiSeq - 100x100 Paired End	Lane	$2,700	avg 120-150 million paired end reads
HiSeq - Additional 40 bases	Lane	$330	Add to above base prices for longer reads

HiSeq (Rapid)

Item	Unit	Price Per Unit	Notes
HiSeqRapid - 50 bp Single Read	full flowcell (2 lanes)	$1,680	avg ~160-200 million reads
HiSeqRapid - 40 bp Single Read	1 lane	$1,140	avg ~80-100 million reads
HiSeqRapid - 50x50 Paired End	full flowcell (2 lanes)	$2,500	avg ~160-200 million paired end reads
HiSeqRapid - 50x50 Paired End	1 lane	$1,500	avg ~80-100 million paired end reads
HiSeqRapid - 100x100 Paired End	full flowcell (2 lanes)	$3,250	avg ~160-200 million paired end reads
HiSeqRapid - 100x100 Paired End	1 lane	$1,850	avg ~80-100 million paired end reads
HiSeqRapid - 250x250 Paired End	full flowcell (2 lanes)	$5,550	avg ~160-200 million paired end reads
HiSeqRapid - Add DUAL mode	full flowcell (2 lanes)	$450	ONLY required if running different samples in each lane
HiSeqRapid - Additional 50 bases	full flowcell (2 lanes)	$500	Add to above base prices for longer reads up to 250x250

Library Prep

Item	Unit	Price Per Unit	Notes
TruSeq ChIP DNA	Sample	$200
Swift ChIP DNA	Sample	$200	Optimized for low input ChIP
TruSeq Nano gDNA	Sample	$200	Genomic DNA >100ng
TruSeq Stranded mRNA	Sample	$200	Basic RNA Expression Analysis
TruSeq Stranded RiboZero	Sample	$250	Replaces polyA selection with a ribozomal RNA depletion
Clontech UltraLow RNA	Sample	$400	For low input RNA down to single cell
Nextera genomic DNA	Sample	$200	"Tagmentation" prep from intact gDNA (50ng)
Sample Multiplexing	Sample	$10	Mixing samples for multiplex run
Quality Control Only	Sample	$40	Bioanalyzer and qPCR

Quality Control

Item	Unit	Price Per Unit	Notes
Bioanalyzer	Sample	$15	Sizing and purity analysis
qPCR	Sample	$25	Quantification
Sample Multiplexing	Sample	$10	Mixing samples for multiplex run

Microarray Services

Prices for sample processing do not include cost of arrays

Affymetrix Array Processing

Item	Unit	Price Per Unit	Notes
Full Service Expression Analysis - 3'IVT or WT	Sample	$325	Not including cost of Array
Contact GTC or Affymetrix for array prices	Array	$150-$400	Array only

Agilent Array Processing

Item	Unit	Price Per Unit	Notes
Full Service Expression Analysis (Single Channel)	Sample	$325	Not including cost of array
Full Service Expression Analysis (Two Samples - Dual Channel)	Two Samples	$525	Not including cost of array
Contact GTC or Agilent for Array pricing	Array	$$

nCounter Analysis System (NanoString)

Item	Unit	Price Per Unit	Notes
Equipment Use	Cartridge	$250	12 samples per cartridge
Reagents	Cartridge	$280.50*	*Does NOT include CodeSet
Labor-Hyb	Cartridge	$150.00	-
Labor-miRNA	Sample	$25.00	-

Bioanalyzer - RNA, DNA and Protein analysis

Item	Unit	Price Per Unit	Notes
RNA or DNA	Sample	$15	Pico, Nano, DNA or hsDNA

Quantitative PCR - QuantStudio 6

Item	Unit	Price Per Unit	Notes
SYBR green / Taq Man Master mix	uL	7 cents	-
Optical Plate	Plate	$7.00	-
Equipment Time (2 hour time block)	Plate	$30.00	Sign-up Required