Pricing

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

Illumina Sequencing

There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".

In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 120-150 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.

In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to two weeks in standard mode. Rapid runs cost approximately 50% more than standard mode runs for similar amounts of data.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

HiSeq (Std)

Item	Unit	Price Per Unit	Notes
HiSeq - 40 bp Single Read	Lane	$1,085	avg 120-130 million reads
HiSeq - 40x40 Paired End	Lane	$1,625	avg 120-130 million paired end reads
HiSeq - 75x75 Paired End	Lane	$2,155	avg 120-130 million paired end reads
HiSeq - 100x100 Paired End	Lane	$2,525	avg 120-130 million paired end reads
HiSeq - Additional 40 bases	Lane	$300	Add to above base prices for longer reads

HiSeq (Rapid)

Item	Unit	Price Per Unit	Notes
HiSeq2500 - 50 bp Single Read	Sample	$1,600	avg 100-120 million reads
HiSeq2500 - 40x40 Paired End	Sample	$2,400	avg 100-120 million paired end reads
HiSeq2500 - 100x100 Paired End	Sample	$3,100	avg 100-120 million paired end reads
HiSeq2500 - 250x250 Paired End	Sample	$5,300	avg 100-120 million paired end reads
HiSeq2500 - Additional 50 bases	Sample	$500	Add to above base prices for longer reads up to 250x250
HiSeq2500 - Add DUAL mode	Sample	$440	For running different samples in each lane

Library Prep

Item	Unit	Price Per Unit	Notes
DNA-Seq Library prep - Basic	Sample	$200	Automated protocol suitable for genomic DNA
DNA-Seq Library prep - Premium	Sample	$275	Manual Protocol ideal for ChIP-seq and other low-input applications
RNA-Seq Library prep - Basic	Sample	$200	Automated protocol, requires minimum 100ng total RNA, single stranded
RNA-Seq Library prep - Premium	Sample	$275	Manual Protocol, uses Illumina "TruSeq" kits
RNA-Seq add RiboZero	Sample	$165	Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)
Sample Multiplexing	Sample	$10	Mixing samples for multiplex run
Quality Control Only	Sample	$40	Bioanalyzer and qPCR

Quality Control

Item	Unit	Price Per Unit	Notes
Bioanalyzer	Sample	$15	Sizing and purity analysis
qPCR	Sample	$25	Quantification
Sample Multiplexing	Sample	$10	Mixing samples for multiplex run

Microarray Services

Prices for sample processing do not include cost of arrays

Affymetrix Array Processing

Item	Unit	Price Per Unit	Notes
Full Service Expression Analysis - 3'IVT or WT	Sample	$325	Not including cost of Array
Contact GTC or Affymetrix for array prices	Array	$150-$400	Array only

Agilent Array Processing

Item	Unit	Price Per Unit	Notes
Full Service Expression Analysis (Single Channel)	Sample	$325	Not including cost of array
Full Service Expression Analysis (Two Samples - Dual Channel)	Two Samples	$525	Not including cost of array
Contact GTC or Agilent for Array pricing	Array	$$

nCounter Analysis System (NanoString)

Item	Unit	Price Per Unit	Notes
Equipment Use	Cartridge	$190	12 samples per cartridge
Reagents	Sample	$23.00*	*Does NOT include CodeSet
Labor	Cartridge	$105.00	-

Bioanalyzer - RNA, DNA and Protein analysis

Item	Unit	Price Per Unit	Notes
RNA or DNA	Sample	$15	Pico, Nano, DNA or hsDNA

Quantitative PCR - ABI 7900

Item	Unit	Price Per Unit	Notes
SYBR green / Taq Man Master mix	uL	7 cents	-
Optical Plate	Plate	$7.00	-
Equipment Time (2 hour time block)	Plate	$30.00	Sign-up Required