Pricing

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.

Illumina Sequencing

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.

GAII

Item	Unit	Price Per Unit	Notes
GAII - 36 bp Single Read	Lane	$810	20-30 million reads
GAII - 36x36 Paired End	Lane	$1,450	20-30 million paired-end reads
GAII - Additional 36 bases	Lane	$300	Add to above base prices for longer reads

HiSeq (Std)

Item	Unit	Price Per Unit	Notes
HiSeq - 40 bp Single Read	Lane	$1,060	avg 120-130 million reads
HiSeq - 40x40 Paired End	Lane	$1,575	avg 120-130 million paired end reads
HiSeq - 75x75 Paired End	Lane	$2,100	avg 120-130 million paired end reads
HiSeq - 100x100 Paired End	Lane	$2,475	avg 120-130 million paired end reads
HiSeq - Additional 40 bases	Lane	$300	Add to above base prices for longer reads

HiSeq (Rapid)

Item	Unit	Price Per Unit	Notes
HiSeq2500 - 50 bp Single Read	Sample	$1,700	avg 100-120 million reads
HiSeq2500 - 50x50 Paired End	Sample	$2,500	avg 100-120 million paired end reads
HiSeq2500 - 100x100 Paired End	Sample	$3,240	avg 100-120 million paired end reads
HiSeq2500 - Additional 50 bases	Sample	$500	Add to above base prices for longer reads up to 150x150
HiSeq2500 - Add DUAL mode	Sample	$440	For running different samples in each lane

Library Prep

Item	Unit	Price Per Unit	Notes
DNA-Seq Library prep - Basic	Sample	$200	Automated protocol suitable for genomic DNA
DNA-Seq Library prep - Premium	Sample	$275	Manual Protocol ideal for ChIP-seq and other low-input applications
RNA-Seq Library prep - Basic	Sample	$200	Automated protocol, requires minimum 100ng total RNA, single stranded
RNA-Seq Library prep - Premium	Sample	$275	Manual Protocol, uses Illumina "TruSeq" kits
RNA-Seq add RiboZero	Sample	$165	Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)
Sample Multiplexing	Sample	$10	Mixing samples for multiplex run
Quality Control Only	Sample	$40	Bioanalyzer and qPCR

Quality Control

Item	Unit	Price Per Unit	Notes
Bioanalyzer	Sample	$15	Sizing and purity analysis
qPCR	Sample	$25	Quantification
Sample Multiplexing	Sample	$10	Mixing samples for multiplex run

Microarray Services

Prices for sample processing do not include cost of arrays

Affymetrix Array Processing

Item	Unit	Price Per Unit	Notes
Affymetrix Gene ST or PrimeView arrays	Array	~$175	Array only
Affymetrix Genome (U133 or 430) 2.0	Array	~$400	Array only
Standard Expression Processing	Sample	~$500	all Affymetrix certified reagents
Prokaryotic Protocol	Sample	~$450	-

Agilent Array Processing

Item	Unit	Price Per Unit	Notes
4x44k multiplex Arrays	Slide (array)	~$700/slide($175/array)	Arrays only - 4 arrays per slide
8x60k multiplex Arrays	Slide (array)	~$1,300/slide($162/array)	Arrays only - 8 arrays per slide
Standard Expression Processing	Sample	~$350	One sample, single channel
ChIP-on-Chip/CGH Processing	Sample	~$500	Two samples, dual channel

nCounter Analysis System (NanoString)

Item	Unit	Price Per Unit	Notes
Equipment Use	Cartridge	$190	12 samples per cartridge
Reagents	Sample	$23.00*	*Does NOT include CodeSet
Labor	Cartridge	$105.00	-

Bioanalyzer - RNA, DNA and Protein analysis

Item	Unit	Price Per Unit	Notes
RNA or DNA	Sample	$15	Pico, Nano, DNA or hsDNA

Quantitative PCR - ABI 7900

Item	Unit	Price Per Unit	Notes
SYBR green / Taq Man Master mix	uL	7 cents	-
Optical Plate	Plate	$7.00	-
Equipment Time (2 hour time block)	Plate	$30.00	Sign-up Required