Difference between revisions of "Pricing"
From Genome Technology Core (GTC) wiki - Sequencing and Microarray
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{{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}} | {{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}} | ||
{{PricingRow|10X ADT/HTO/CellPlex Add-on|sample|$140|}} | {{PricingRow|10X ADT/HTO/CellPlex Add-on|sample|$140|}} | ||
Revision as of 12:29, 18 July 2023
Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.
Contents
Illumina Sequencing
There are three options for Illumina sequencing. NovaSeq and MiSeq.
Prices include basic data processing including base calls, quality scores and alignment (if requested).
Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.
NovaSeq (Single lane submissions accepted for standard read lengths)
| Item | Read Length | Unit | Price Per Unit | Notes |
| NovaSeqSP | 100 Single End or 50x50 Paired-End | 1 lane | $1,850 | ~650–800 million reads per flowcell ~325-400 million reads per lane (x2 for Paired-End) |
| 200 Single End or 100x100 Paired-End | $2,245 | |||
| 300 Single End or 150x150 Paired-End | $2,620 | |||
| 500 Single End or 250x250 Paired-End | $3,510 | |||
| NovaSeqS1 | 100 Single End or 50x50 Paired-End | 1 lane | $2,920 | ~1.3–1.6 billion reads per flowcell ~650–800 million reads per lane (x2 for Paired-End) |
| 200 Single End or 100x100 Paired-End | $3,730 | |||
| 300 Single End or 150x150 Paired-End | $4,065 | |||
| NovaSeqS2 | 100 Single End or 50x50 Paired-End | 1 lane | $5,200 | ~3.3–4.1 billion reads per flowcell ~1.65-2.05 billion reads per lane (x2 for Paired-End) |
| 200 Single End or 100x100 Paired-End | $6,480 | |||
| 300 Single End or 150x150 Paired-End | $6,750 | |||
| NovaSeqS4 | 50 Single End | 1 lane | $3,780 | ~8-10 billion reads per flowcell ~2-2.5 billion reads per lane (x2 for Paired-End) |
| 300 Single End or 150x150 Paired-End | $5,400 |
MiSeq
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| MiSeq v2 70 Single End or 30x30 Paired End | Flowcell | $1,320 | 10 million reads |
| MiSeq v3 150 Single End or 75x75 Paired End | Flowcell | $1,430 | 20 million reads |
| MiSeq v2 300 Single End or 150x150 Paired End | Flowcell | $1,595 | 10 million reads |
| MiSeq v2 500 Single End or 250x250 Paired End | Flowcell | $1,760 | 10 million reads |
| MiSeq v3 600 Single End or 300x300 Paired End | Flowcell | $2,200 | 20 million reads |
RNA Library Prep
Standard (Manual)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12 | Sample | $200 | |
| IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+ | Sample | $150 | |
| KAPA Hyper Prep Ribo Zero, samples 1-12 | Sample | $250 | |
| KAPA Hyper Prep Ribo Zero, samples 13+ | Sample | $200 | |
| Agilent SureSelect All Exon | Sample | $650 | |
| NEBNext Small RNA | Sample | $200 |
Highthroughput (96 well Plate Based)
| Item | Unit | Price Per Unit | Notes |
| HT mRNA library prep (IDT xGen RNA) | 48 Samples | $3600 | Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.
Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed. Libraries are pooled by FA quantification, expect variation in read balancing. |
| 49-72 samples | $5400 | ||
| 96 samples | $7200 |
Single Cell (10X and others)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| 10X Single Cell 3’ GEX | sample | $2560 | |
| 10X Single Cell 5’ GEX | sample | $2560 | |
| 10X VDJ Add-on | sample | $230 | Can be added onto the 10X 5' GEX Only |
| 10X ADT/HTO/CellPlex Add-on | sample | $140 | |
| 10X Multiome Assay (ATAC + 3') | sample | $4200 | |
| 10X Flex (Fixed Cell) | sample | $2800 | |
| Plexwell Rapid Single Cell - one 96 well plate | 96 well plate | $5060 | 1 cell per well |
| Plexwell Rapid Single Cell - 1 of 4 96 well plates | 96 well plate | $3800 | 1 cell per well |
DNA Library Prep
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Swift ChIP | sample | $200 | Optimized for low input ChIP |
| Nextera gDNA (Genomic, ATAC-Seq) | sample | $200 | "Tagmentation" prep from intact gDNA (50ng) |
| Illumina DNA Prep (Genomic) | sample | $200 | Genomic DNA >100ng |
RNA Isolation
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| RNA-miRNeasy Total RNA Isolation | sample | $100 | |
| RNA - MagMax mirVana RNA Isolation | sample | $30 |
Quality Control
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Bioanalyzer | Sample | $15 | Sizing and purity analysis |
| qPCR | Sample | $25 | Quantification |
| Sample Multiplexing | Sample | $10 | Mixing samples for multiplex run |
Bioanalyzer - RNA, DNA and Protein analysis
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| RNA or DNA | Sample | $15 | Pico, Nano, DNA or hsDNA |
Quantitative PCR - QuantStudio 6
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| SYBR green / Taq Man Master mix | uL | 7 cents | - |
| Optical Plate | Plate | $7 | - |
| Equipment Time (2 hour time block) | Plate | $30 | Sign-up Required |
