Difference between revisions of "Pricing"
From Genome Technology Core (GTC) wiki - Sequencing and Microarray
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Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services. | Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services. | ||
| − | === NovaSeq === | + | === NovaSeq (Single lane submissions accepted for standard read lengths) === |
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Revision as of 05:33, 7 July 2022
Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.
Contents
Illumina Sequencing
There are three options for Illumina sequencing. NovaSeq, MiSeq, HiSeq "Standard" and HiSeq "Rapid".
Prices include basic data processing including base calls, quality scores and alignment (if requested).
Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.
NovaSeq (Single lane submissions accepted for standard read lengths)
| Item | Read Length | Unit | Price Per Unit | Notes |
| NovaSeqSP | 100 Single End or 50x50 Paired-End | Flow Cell (2 Lanes) | $3,425 | ~650–800 million reads per flowcell ~325-400 million reads per lane (x2 for Paired-End) |
| 200 Single End or 100x100 Paired-End | $4,150 | |||
| 300 Single End or 150x150 Paired-End | $4,850 | |||
| 500 Single End or 250x250 Paired-End | $6,500 | |||
| NovaSeqS1 | 100 Single End or 50x50 Paired-End | Flow Cell (2 Lanes) | $5,400 | ~1.3–1.6 billion reads per flowcell ~650–800 million reads per lane (x2 for Paired-End) |
| 200 Single End or 100x100 Paired-End | $6,900 | |||
| 300 Single End or 150x150 Paired-End | $7,525 | |||
| NovaSeqS2 | 100 Single End or 50x50 Paired-End | Flow Cell (2 Lanes) | $9,625 | ~3.3–4.1 billion reads per flowcell ~1.65-2.05 billion reads per lane (x2 for Paired-End) |
| 200 Single End or 100x100 Paired-End | $12,000 | |||
| 300 Single End or 150x150 Paired-End | $12,750 | |||
| NovaSeqS4 | 35 Single End | Flow Cell (4 Lanes) | $14,400 | ~8-10 billion reads per flowcell ~2-2.5 billion reads per lane (x2 for Paired-End) |
| 300 Single End or 150x150 Paired-End | $20,000 | |||
| Add XP protocol - SP,S1,S2 | Any Read Length | Flow Cell | $400 | Allows different library pools in each lane |
| Add XP protocol - S4 | Any Read Length | Flow Cell | $1,200 | Allows different library pools in each lane |
MiSeq -- 20 million reads
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| MiSeq v3 150 Single End or 75x75 Paired End | Flowcell | $1,485 | |
HiSeq (Std a.k.a. "High Output" mode) -- At least 150-200 million reads per lane
Standard mode require 8 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| HiSeq - 50 bp Single Read | Lane | $1,200 | |
| HiSeq - Additional 70 bases | Lane | $400 | Add to above base prices for longer reads |
HiSeq (Rapid) -- At least 100 to 150 million reads per lane (Two lanes per Flow Cell)
In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250, and complete more quickly. For example - a 100x100 run in Rapid mode completes in 2-3 days vs up to 7 days in Standard mode.
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| HiSeqRapid - 50 bp Single Read | full flowcell (2 lanes) | $2,300 | |
| HiSeqRapid - 28x40 10X | full flowcell (2 lanes) | $2,800 | ONLY FOR 10X LIBRARIES |
| HiSeqRapid - 60x60 Paired End | full flowcell (2 lanes) | $3,400 | |
| HiSeqRapid - 100x100 Paired End | full flowcell (2 lanes) | $4,350 | |
| HiSeqRapid - 250x250 Paired End | full flowcell (2 lanes) | $7,200 | |
| HiSeqRapid - Add DUAL mode | full flowcell (2 lanes) | $460 | ONLY required if running different samples in each lane |
| HiSeqRapid - Additional 70 bases | full flowcell (2 lanes) | $560 | Add to above base prices for longer reads up to 250x250 |
Library Prep
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Swift ChIP DNA (ChIP-Seq) | Sample | $200 | Optimized for low input ChIP |
| Illumina DNA (Genomic) | Sample | $200 | Genomic DNA >100ng |
| Nextera gDNA (Genomic, ATAC-Seq) | Sample | $200 | "Tagmentation" prep from intact gDNA (50ng) |
| KAPA Hyper Prep - Stranded (RNA-Seq) | Sample | $200 | |
| KAPA Ribo Erase (RNA-Seq) | Sample | $250 | |
| Clontech UltraLow RNA (RNA-Seq) | Sample | $300 | For low input RNA down to single cell |
| Clontech Stranded Total RNA (RNA-Seq) | Sample | $250 | - |
| NEBNext Small RNA (microRNA) | Sample | $200 | - |
| 10X Single Cell 3’ Solution Library prep (RNA-Seq) | Sample | $2,205 | Sorting and library prep for up to 10,000 cells |
| 10X Single Cell 5’ Solution Library prep (RNA-Seq) | Sample | $2,363 | Sorting and library prep for up to 10,000 cells |
| SMART-seq v2 Library prep (RNA-Seq) | 96-well Plate | $2,500 |
| Click here for more info about Library Prep options |
Quality Control
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Bioanalyzer | Sample | $15 | Sizing and purity analysis |
| qPCR | Sample | $25 | Quantification |
| Sample Multiplexing | Sample | $10 | Mixing samples for multiplex run |
nCounter Analysis System (NanoString)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Reagents | Cartridge | ---- | Reagents and Codesets Purchase From Nanostring |
| Equipment Use | Cartridge | $250 | 12 samples per cartridge |
| Labor-Hyb | Cartridge | $150 | - |
| Labor-miRNA | Sample | $25 | Addon for miRNA protocol only |
Bioanalyzer - RNA, DNA and Protein analysis
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| RNA or DNA | Sample | $15 | Pico, Nano, DNA or hsDNA |
Quantitative PCR - QuantStudio 6
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| SYBR green / Taq Man Master mix | uL | 7 cents | - |
| Optical Plate | Plate | $7 | - |
| Equipment Time (2 hour time block) | Plate | $30 | Sign-up Required |
