Difference between revisions of "Pricing"

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{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,700|avg 100-120 million reads}}
 
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,700|avg 100-120 million reads}}
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{{PricingRow|HiSeq2500 - 50x50 Paired End|Sample|$2,500|avg 100-120 million paired end reads}}
 
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,240|avg 100-120 million paired end reads}}
 
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,240|avg 100-120 million paired end reads}}
 
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 150x150}}
 
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 150x150}}

Revision as of 11:34, 28 April 2014

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.

The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.

Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.


GAII

Item Unit Price Per Unit Notes
GAII - 36 bp Single Read Lane $810 20-30 million reads
GAII - 36x36 Paired End Lane $1,450 20-30 million paired-end reads
GAII - Additional 36 bases Lane $300 Add to above base prices for longer reads


HiSeq (Std)

Item Unit Price Per Unit Notes
HiSeq - 40 bp Single Read Lane $1,060 avg 120-130 million reads
HiSeq - 40x40 Paired End Lane $1,575 avg 120-130 million paired end reads
HiSeq - 75x75 Paired End Lane $2,100 avg 120-130 million paired end reads
HiSeq - 100x100 Paired End Lane $2,475 avg 120-130 million paired end reads
HiSeq - Additional 40 bases Lane $300 Add to above base prices for longer reads


HiSeq (Rapid)

Item Unit Price Per Unit Notes
HiSeq2500 - 50 bp Single Read Sample $1,700 avg 100-120 million reads
HiSeq2500 - 50x50 Paired End Sample $2,500 avg 100-120 million paired end reads
HiSeq2500 - 100x100 Paired End Sample $3,240 avg 100-120 million paired end reads
HiSeq2500 - Additional 50 bases Sample $500 Add to above base prices for longer reads up to 150x150
HiSeq2500 - Add DUAL mode Sample $440 For running different samples in each lane


Library Prep

Item Unit Price Per Unit Notes
DNA-Seq Library prep - Basic Sample $200 Automated protocol suitable for genomic DNA
DNA-Seq Library prep - Premium Sample $275 Manual Protocol ideal for ChIP-seq and other low-input applications
RNA-Seq Library prep - Basic Sample $200 Automated protocol, requires minimum 100ng total RNA, single stranded
RNA-Seq Library prep - Premium Sample $275 Manual Protocol, uses Illumina "TruSeq" kits
RNA-Seq add RiboZero Sample $165 Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)
Sample Multiplexing Sample $10 Mixing samples for multiplex run
Quality Control Only Sample $40 Bioanalyzer and qPCR


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Microarray Services

Prices for sample processing do not include cost of arrays

Affymetrix Array Processing

Item Unit Price Per Unit Notes
Affymetrix Gene ST or PrimeView arrays Array ~$175 Array only
Affymetrix Genome (U133 or 430) 2.0 Array ~$400 Array only
Standard Expression Processing Sample ~$500 all Affymetrix certified reagents
Prokaryotic Protocol Sample ~$450 -


Agilent Array Processing

Item Unit Price Per Unit Notes
4x44k multiplex Arrays Slide (array) ~$700/slide($175/array) Arrays only - 4 arrays per slide
8x60k multiplex Arrays Slide (array) ~$1,300/slide($162/array) Arrays only - 8 arrays per slide
Standard Expression Processing Sample ~$350 One sample, single channel
ChIP-on-Chip/CGH Processing Sample ~$500 Two samples, dual channel


nCounter Analysis System (NanoString)

Item Unit Price Per Unit Notes
Equipment Use Cartridge $190 12 samples per cartridge
Reagents Sample $23.00* *Does NOT include CodeSet
Labor Cartridge $105.00 -


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - ABI 7900

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7.00 -
Equipment Time (2 hour time block) Plate $30.00 Sign-up Required