Difference between revisions of "LibraryPrep"
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=== KAPA Hyperprep with Ribo-depletion === | === KAPA Hyperprep with Ribo-depletion === | ||
| − | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome analysis. Data is strand-specific. 100-1000ng total RNA (more is better) | + | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better) |
=== Clonetech SMART-Seq v4 Ultra-low === | === Clonetech SMART-Seq v4 Ultra-low === | ||
Revision as of 12:46, 5 February 2020
Contents
Library prep
RNA
KAPA Hyperprep mRNA
The most common RNA kit option. Best for standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.
KAPA Hyperprep with Ribo-depletion
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)
Clonetech SMART-Seq v4 Ultra-low
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.
Clonetech SMARTer PICO
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.
NEBnext small RNA
For microRNA and other small RNA. 100-1000ng total RNA.
DNA
Swift 2S
For ChIP and other low input DNA. For ChIP samples the input is enriched and purified dsDNA. There is no lower limit for input but odds of failure goes up with lower input. >10ng is generally fine. Nextera gDNA: “Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
