Difference between revisions of "Pricing"
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{{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}} | {{PricingRow|Clontech UltraLow RNA (RNA-Seq)|Sample|$300|For low input RNA down to single cell}} | ||
{{PricingRow|Clontech Stranded Total RNA Pico (RNA-Seq)|Sample|$250|-}} | {{PricingRow|Clontech Stranded Total RNA Pico (RNA-Seq)|Sample|$250|-}} | ||
| + | {{PricingRow|NEBNext Small RNA (microRNA)|Sample|$200|-}} | ||
{{PricingRow|10X Single Cell 3’ Solution Library prep (RNA-Seq)|Sample|$1800|}} | {{PricingRow|10X Single Cell 3’ Solution Library prep (RNA-Seq)|Sample|$1800|}} | ||
| − | {{ | + | {{PricingLastRow|SMART-seq v2 Library prep (96 well plate) (RNA-Seq)|Plate|$2400|}} |
| − | |||
<br> | <br> | ||
Revision as of 14:50, 14 August 2017
Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.
Contents
Illumina Sequencing
There are two options for Illumina sequencing. HiSeq "Standard" and HiSeq "Rapid".
In Standard mode (a.k.a. "High Output" mode) the HiSeq typically yields 150-200 million reads per lane. Standard mode require 7 full lanes of samples before sequencing can proceed and takes about 1 day for every 25 bases of read length. Note: You do not need to fill out a full flow cell yourself, we will group you with other samples to fill out a run.
In "Rapid" mode, individual samples can be sequenced much more quickly without the need to fill out full flow cells. Read lengths in Rapid mode are also longer, up to 250x250 and can be completed in a day or two compared to up to 10 days in standard mode.
Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.
Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.
HiSeq (Std)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| HiSeq - 40 bp Single Read | Lane | $1,140 | avg 150-200 million reads |
| HiSeq - 40x40 Paired End | Lane | $1,710 | avg 150-200 million paired end reads |
| HiSeq - 75x75 Paired End | Lane | $2,260 | avg 150-200 million paired end reads |
| HiSeq - 100x100 Paired End | Lane | $2,700 | avg 150-200 million paired end reads |
| HiSeq - Additional 40 bases | Lane | $330 | Add to above base prices for longer reads |
HiSeq (Rapid)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| HiSeqRapid - 50 bp Single Read | full flowcell (2 lanes) | $1,680 | avg 200-280 million reads |
| HiSeqRapid - 40 bp Single Read | 1 lane | $1,140 | avg 100-140 million reads |
| HiSeqRapid - 50x50 Paired End | full flowcell (2 lanes) | $2,500 | avg 200-280 million paired end reads |
| HiSeqRapid - 50x50 Paired End | 1 lane | $1,500 | avg 100-140 million paired end reads |
| HiSeqRapid - 100x100 Paired End | full flowcell (2 lanes) | $3,250 | avg 200-280 million paired end reads |
| HiSeqRapid - 100x100 Paired End | 1 lane | $1,850 | avg 100-140 million paired end reads |
| HiSeqRapid - 250x250 Paired End | full flowcell (2 lanes) | $5,550 | avg 200-280 million paired end reads |
| HiSeqRapid - Add DUAL mode | full flowcell (2 lanes) | $450 | ONLY required if running different samples in each lane |
| HiSeqRapid - Additional 50 bases | full flowcell (2 lanes) | $500 | Add to above base prices for longer reads up to 250x250 |
Library Prep
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Swift ChIP DNA (ChIP-Seq) | Sample | $200 | Optimized for low input ChIP |
| TruSeq Nano gDNA (Genomic) | Sample | $200 | Genomic DNA >100ng |
| Nextera gDNA (Genomic, ATAC-Seq) | Sample | $200 | "Tagmentation" prep from intact gDNA (50ng) |
| TruSeq Stranded mRNA (RNA-Seq) | Sample | $200 | Basic RNA Expression Analysis |
| TruSeq Stranded RiboZero (RNA-Seq) | Sample | $250 | Replaces polyA selection with a ribozomal RNA depletion |
| Clontech UltraLow RNA (RNA-Seq) | Sample | $300 | For low input RNA down to single cell |
| Clontech Stranded Total RNA Pico (RNA-Seq) | Sample | $250 | - |
| NEBNext Small RNA (microRNA) | Sample | $200 | - |
| 10X Single Cell 3’ Solution Library prep (RNA-Seq) | Sample | $1800 | |
| SMART-seq v2 Library prep (96 well plate) (RNA-Seq) | Plate | $2400 |
Quality Control
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Bioanalyzer | Sample | $15 | Sizing and purity analysis |
| qPCR | Sample | $25 | Quantification |
| Sample Multiplexing | Sample | $10 | Mixing samples for multiplex run |
Microarray Services
Prices for sample processing do not include cost of arrays
Affymetrix Array Processing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Full Service Expression Analysis - 3'IVT or WT | Sample | $325 | Not including cost of Array |
| Contact GTC or Affymetrix for array prices | Array | $150-$400 | Array only |
Agilent Array Processing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Full Service Expression Analysis (Single Channel) | Sample | $325 | Not including cost of array |
| Full Service Expression Analysis (Two Samples - Dual Channel) | Two Samples | $525 | Not including cost of array |
| Contact GTC or Agilent for Array pricing | Array | $$ |
nCounter Analysis System (NanoString)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Equipment Use | Cartridge | $250 | 12 samples per cartridge |
| Reagents | Cartridge | $280.50* | *Does NOT include CodeSet |
| Labor-Hyb | Cartridge | $150.00 | - |
| Labor-miRNA | Sample | $25.00 | - |
Bioanalyzer - RNA, DNA and Protein analysis
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| RNA or DNA | Sample | $15 | Pico, Nano, DNA or hsDNA |
Quantitative PCR - QuantStudio 6
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| SYBR green / Taq Man Master mix | uL | 7 cents | - |
| Optical Plate | Plate | $7.00 | - |
| Equipment Time (2 hour time block) | Plate | $30.00 | Sign-up Required |
