Difference between revisions of "Pricing"
From Genome Technology Core (GTC) wiki - Sequencing and Microarray
(→Illumina Sequencing) |
(→Illumina Sequencing) |
||
| Line 19: | Line 19: | ||
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}} | {{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}} | ||
| − | {{PricingRow|DNA-Seq Library prep Basic|Sample|$200|Automated protocol suitable for genomic DNA}} | + | {{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}} |
| − | {{PricingRow|DNA-Seq Library prep Premium |Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}} | + | {{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}} |
| − | {{PricingRow|RNA-Seq Library prep Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}} | + | {{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}} |
| − | {{PricingRow|RNA-Seq Library prep Premium |Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}} | + | {{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}} |
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly A mRNA isolation}} | {{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly A mRNA isolation}} | ||
| − | |||
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}} | {{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}} | ||
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}} | {{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}} | ||
Revision as of 15:06, 24 July 2013
Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.
Contents
Illumina Sequencing
There are two options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The new HiSeq typically yields 70-130 million reads per lane. Prices include sample quality control for one sample and basic data processing including base calls, quality scores and alignment. Sequencing pricing based on 7 samples per flow cell plus a control lane.
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| GAII - 36 bp Single Read | Lane | $810 | 20-30 million reads |
| GAII - 36x36 Paired End | Lane | $1,450 | 20-30 million paired-end reads |
| GAII - Additional 36 bases | Lane | $300 | Add to above base prices for longer reads |
| HiSeq - 40 bp Single Read | Lane | $1,060 | 70-130 million reads |
| HiSeq - 40x40 Paired End | Lane | $1,575 | 70-130 million paired end reads |
| HiSeq - 75x75 Paired End | Lane | $2,100 | 70-130 million paired end reads |
| HiSeq - 100x100 Paired End | Lane | $2,475 | 70-130 million paired end reads |
| HiSeq - Additional 40 bases | Lane | $300 | Add to above base prices for longer reads |
| HiSeq2500 - 50 bp Single Read | Sample | $1,700 | 70-130 million reads |
| HiSeq2500 - 100x100 Paired End | Sample | $3,240 | 70-130 million paired end reads |
| HiSeq2500 - Additional 50 bases | Sample | $500 | Add to above base prices for longer reads up to 150x150 |
| HiSeq2500 - Add DUAL mode | Sample | $440 | For running different samples in each lane |
| DNA-Seq Library prep - Basic | Sample | $200 | Automated protocol suitable for genomic DNA |
| DNA-Seq Library prep - Premium | Sample | $275 | Manual Protocol ideal for ChIP-seq and other low-input applications |
| RNA-Seq Library prep - Basic | Sample | $200 | Automated protocol, requires minimum 100ng total RNA, single stranded |
| RNA-Seq Library prep - Premium | Sample | $275 | Manual Protocol, uses Illumina "TruSeq" kits |
| RNA-Seq add RiboZero | Sample | $165 | Uses Epicenter RiboZero kit instead of poly A mRNA isolation |
| Sample Multiplexing | Sample | $10 | Mixing samples for multiplex run |
| Quality Control Only | Sample | $40 | Bioanalyzer and qPCR |
Microarray Services
Prices for sample processing do not include cost of arrays
Affymetrix Array Processing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Affymetrix Gene ST or PrimeView arrays | Array | ~$175 | Array only |
| Affymetrix Genome (U133 or 430) 2.0 | Array | ~$400 | Array only |
| Standard Expression Processing | Sample | ~$500 | all Affymetrix certified reagents |
| Prokaryotic Protocol | Sample | ~$450 | - |
Agilent Array Processing
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| 4x44k multiplex Arrays | Slide (array) | ~$700/slide($175/array) | Arrays only - 4 arrays per slide |
| 8x60k multiplex Arrays | Slide (array) | ~$1,300/slide($162/array) | Arrays only - 8 arrays per slide |
| Standard Expression Processing | Sample | ~$350 | One sample, single channel |
| ChIP-on-Chip/CGH Processing | Sample | ~$500 | Two samples, dual channel |
nCounter Analysis System (NanoString)
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Equipment Use | Cartridge | $190 | 12 samples per cartridge |
| Reagents | Sample | $23.00* | *Does NOT include CodeSet |
| Labor | Cartridge | $105.00 | - |
Bioanalyzer - RNA, DNA and Protein analysis
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| RNA or DNA | Sample | $15 | Pico, Nano, DNA or hsDNA |
Quantitative PCR - ABI 7900
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| SYBR green / Taq Man Master mix | uL | 6.5 cents | - |
| Optical Plate | Plate | $7.00 | - |
| Equipment Time (2 hour time block) | Plate | $30.00 | Sign-up Required |
Highthroughput Screening - Cellomics
| Item | Unit | Price Per Unit | Notes |
|---|---|---|---|
| Equipment Time (Internal) | Hourly | $25.00 | Sign-up Required |
| Equipment Time (External) | Hourly | $75.00 | Sign-up Required |
