Difference between revisions of "LibraryPrep"
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= Library prep = | = Library prep = | ||
== RNA == | == RNA == | ||
| − | === | + | === Watchmaker for mRNA === |
| − | + | For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA. | |
| − | === | + | === Watchmaker for Ribodepletion === |
| − | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. | + | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. |
| + | |||
| + | === NEBnext small RNA === | ||
| + | For microRNA and other small RNA. 100-1000ng total RNA. | ||
=== Clonetech SMART-Seq v4 Ultra-low === | === Clonetech SMART-Seq v4 Ultra-low === | ||
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA. | For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA. | ||
| − | === Clonetech SMARTer PICO === | + | === IDT/xGen for mRNA (Supported Only For Legacy Projects) === |
| + | For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA. | ||
| + | |||
| + | === KAPA Hyperprep mRNA (Supported Only For Legacy Projects) === | ||
| + | For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA. | ||
| + | |||
| + | === KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects) === | ||
| + | Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better) | ||
| + | |||
| + | === Clonetech SMARTer PICO (Supported Only For Legacy Projects) === | ||
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA. | For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA. | ||
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| − | |||
| − | |||
== DNA == | == DNA == | ||
| − | |||
| − | |||
=== Nextera gDNA === | === Nextera gDNA === | ||
| − | “Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications | + | “Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications. |
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Latest revision as of 18:08, 10 July 2024
Contents
- 1 Library prep
- 1.1 RNA
- 1.1.1 Watchmaker for mRNA
- 1.1.2 Watchmaker for Ribodepletion
- 1.1.3 NEBnext small RNA
- 1.1.4 Clonetech SMART-Seq v4 Ultra-low
- 1.1.5 IDT/xGen for mRNA (Supported Only For Legacy Projects)
- 1.1.6 KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
- 1.1.7 KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
- 1.1.8 Clonetech SMARTer PICO (Supported Only For Legacy Projects)
- 1.2 DNA
- 1.1 RNA
Library prep
RNA
Watchmaker for mRNA
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
Watchmaker for Ribodepletion
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis.
NEBnext small RNA
For microRNA and other small RNA. 100-1000ng total RNA.
Clonetech SMART-Seq v4 Ultra-low
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.
IDT/xGen for mRNA (Supported Only For Legacy Projects)
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
KAPA Hyperprep mRNA (Supported Only For Legacy Projects)
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.
KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)
Clonetech SMARTer PICO (Supported Only For Legacy Projects)
For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.
DNA
Nextera gDNA
“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
