Difference between revisions of "LibraryPrep"

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= Library prep =
 
= Library prep =
 
== RNA ==
 
== RNA ==
−
=== KAPA Hyperprep mRNA ===   
+
=== Watchmaker for mRNA ===   
−
The most common RNA kit option. Best for standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.
+
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
  
−
=== KAPA Hyperprep with Ribo-depletion ===
+
=== Watchmaker for Ribodepletion ===
−
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)
+
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis.  
 +
 
 +
=== NEBnext small RNA ===
 +
For microRNA and other small RNA. 100-1000ng total RNA.
  
 
=== Clonetech SMART-Seq v4 Ultra-low ===  
 
=== Clonetech SMART-Seq v4 Ultra-low ===  
 
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.
 
For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.
  
−
=== Clonetech SMARTer PICO ===  
+
=== IDT/xGen for mRNA (Supported Only For Legacy Projects) === 
 +
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.
 +
 
 +
=== KAPA Hyperprep mRNA (Supported Only For Legacy Projects) === 
 +
For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.
 +
 
 +
=== KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects) ===
 +
Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)
 +
 
 +
=== Clonetech SMARTer PICO (Supported Only For Legacy Projects) ===  
 
For low input of degraded RNA.  Data is strand-specific.  10-100ng total RNA.
 
For low input of degraded RNA.  Data is strand-specific.  10-100ng total RNA.
−
 
−
=== NEBnext small RNA ===
 
−
For microRNA and other small RNA. 100-1000ng total RNA.
 
  
 
== DNA ==
 
== DNA ==
−
=== Swift 2S ===
 
−
For ChIP and other low input DNA. For ChIP samples the input is enriched and purified dsDNA. There is no lower limit for input but odds of failure goes up with lower input. >10ng is generally fine.
 
−
Nextera gDNA: “Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
 
  
 
=== Nextera gDNA ===
 
=== Nextera gDNA ===
−
“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
+
“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.
−
 
 
−
== Frequently Asked Questions ==
 
−
Please refer to [[Faq#Bulk_RNA-Seq|frequently asked questions section for FAQ regarding]] library prep options.
 

Latest revision as of 17:08, 10 July 2024

Library prep

RNA

Watchmaker for mRNA

For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.

Watchmaker for Ribodepletion

Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis.

NEBnext small RNA

For microRNA and other small RNA. 100-1000ng total RNA.

Clonetech SMART-Seq v4 Ultra-low

For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA.

IDT/xGen for mRNA (Supported Only For Legacy Projects)

For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 10-500ng total RNA.

KAPA Hyperprep mRNA (Supported Only For Legacy Projects)

For standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA.

KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects)

Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better)

Clonetech SMARTer PICO (Supported Only For Legacy Projects)

For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA.

DNA

Nextera gDNA

“Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications.

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