Difference between revisions of "Pricing"

From Genome Technology Core (GTC) wiki - Sequencing and Microarray
Jump to: navigation, search
(→‎Agilent Array Processing)
 
(181 intermediate revisions by 2 users not shown)
Line 2: Line 2:
  
  
−
= Illumina Sequencing =
+
= Sequencing =
 
 
−
There are three options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The newer HiSeq typically yields 120-150 million reads per lane. The GAII and the HiSeq in "standard" mode require 7 full lanes of samples before sequencing can proceed.
+
There are three options for Illumina sequencing. NovaSeq and MiSeq.
  
−
The HiSeq 2500 upgrade allows for single samples to be run in "Rapid" mode. These runs can be processed much more quickly without the need to fill 7 lanes in a standard flowcell. Read lengths in Rapid mode are also longer, up to 150x150 and can be completed in a day or two compared to up to two weeks in standard mode.
+
Prices include basic data processing including base calls, quality scores and alignment (if requested).  
  
−
Prices include basic data processing including base calls, quality scores and alignment. Sequencing pricing for GAII and HiSeq in standard mode are based on 7 samples per flow-cell plus a control lane.  
+
Please visit the [http://gtc.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
  
−
+
== Short Read (Single lane submissions accepted for standard read lengths) ==
  
−
=== GAII ===
+
=== Illumina NovaSeq ===
 +
 
 +
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 +
|-
 +
| Item || Read Length || Unit || Price Per Unit || Notes
 +
|-
 +
|rowspan="4"|NovaSeqSP
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="4"| 1 lane
 +
|$1,850
 +
|rowspan="4"|~650–800 million reads per flowcell <br>~325-400 million reads per lane <br>(x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$2,245
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$2,620
 +
|-
 +
| 500 Single End or 250x250 Paired-End
 +
|$3,510
 +
|-
 +
|rowspan="3"|NovaSeqS1
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$2,920
 +
|rowspan="3"|~1.3–1.6 billion reads per flowcell <br> ~650–800 million reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$3,730
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$4,065
 +
|-
 +
|rowspan="3"|NovaSeqS2
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$5,200
 +
|rowspan="3"|~3.3–4.1 billion reads per flowcell <br> ~1.65-2.05 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$6,480
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$6,750
 +
|-
 +
|rowspan="2"|NovaSeqS4
 +
| 50 Single End
 +
|rowspan="2"|1 lane
 +
|$3,780
 +
|rowspan="2"|~8-10 billion reads per flowcell <br> ~2-2.5 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$5,400
 +
|-
 +
|}
 +
 
 +
 
 +
<br>
 +
 
 +
=== Element AVITI ===
 +
{{PricingHeader}}
 +
{{PricingRow|150 Single End or 75x75 Paired End|Lane|$1,000| 400 million reads}}
 +
{{PricingRow|300 Single End or 150x150 Paired End|Lane|$1,375| 400 million reads}}
 +
{{PricingLastRow|300x300 Paired End|Flowcell|$3,500| 300 million reads}}
 +
 
 +
 
 +
<br>
 +
 
 +
=== Illumina MiSeq ===
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
+
{{PricingRow|MiSeq v2 70 Single End or 30x30 Paired End|Flowcell|$1,320| 10 million reads}}
−
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
+
{{PricingRow|MiSeq v3 150 Single End or 75x75 Paired End|Flowcell|$1,430| 20 million reads}}
−
{{PricingLastRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
+
{{PricingRow|MiSeq v2 300 Single End or 150x150 Paired End|Flowcell|$1,595| 10 million reads}}
 +
{{PricingRow|MiSeq v2 500 Single End or 250x250 Paired End|Flowcell|$1,760| 10 million reads}}
 +
{{PricingLastRow|MiSeq v3 600 Single End or 300x300 Paired End|Flowcell|$2,200| 20 million reads}}
 +
 
 
<br>
 
<br>
−
=== HiSeq (Std) ===
+
 
 +
== Long Read ==
 +
 
 +
=== Nanopore (P2 Solo/promethION) ===
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,085|avg 120-130 million reads}}
+
{{PricingLastRow|Nanopore|Flowcell|$1200| Upto 50 to 70GB data per flow cell}}
−
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,625|avg 120-130 million paired end reads}}
 
−
{{PricingRow|HiSeq - 75x75 Paired End|Lane|$2,155|avg 120-130 million paired end reads}}
 
−
{{PricingRow|HiSeq - 100x100 Paired End|Lane|$2,525|avg 120-130 million paired end reads}}
 
−
{{PricingRow|HiSeq - Additional 40 bases|Lane|$300|Add to above base prices for longer reads}}
 
  
−
{{PricingLastRow||||}}
 
 
<br>
 
<br>
  
−
=== HiSeq (Rapid) ===
+
=== Pacbio ===
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|HiSeq2500 - 50 bp Single Read|Sample|$1,600|avg 100-120 million reads}}
+
{{PricingLastRow|Pacbio SMRTCell 25 Million|Run|$2900|Around 6 million reads, outsourcing to sister facilities to enable access to the REVIO}}
−
{{PricingRow|HiSeq2500 - 40x40 Paired End|Sample|$2,400|avg 100-120 million paired end reads}}
 
−
{{PricingRow|HiSeq2500 - 100x100 Paired End|Sample|$3,100|avg 100-120 million paired end reads}}
 
−
{{PricingRow|HiSeq2500 - 250x250 Paired End|Sample|$5,300|avg 100-120 million paired end reads}}
 
−
{{PricingRow|HiSeq2500 - Additional 50 bases|Sample|$500|Add to above base prices for longer reads up to 250x250}}
 
−
{{PricingRow|HiSeq2500 - Add DUAL mode|Sample|$440|For running different samples in each lane}}
 
−
{{PricingLastRow||||}}
 
  
 
<br>
 
<br>
  
−
=== Library Prep ===
+
= RNA Library Prep =
 +
=== Standard (Manual) ===
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|DNA-Seq Library prep - Basic|Sample|$200|Automated protocol suitable for genomic DNA}}
+
{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12|Sample|$200|}}
−
{{PricingRow|DNA-Seq Library prep - Premium|Sample|$275|Manual Protocol ideal for ChIP-seq and other low-input applications}}
+
{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+|Sample|$150|}}
−
{{PricingRow|RNA-Seq Library prep - Basic|Sample|$200|Automated protocol, requires minimum 100ng total RNA, single stranded}}
+
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 1-12|Sample|$200|}}
−
{{PricingRow|RNA-Seq Library prep - Premium|Sample|$275|Manual Protocol, uses Illumina "TruSeq" kits}}
+
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 13+|Sample|$150|}}
−
{{PricingRow|RNA-Seq add RiboZero |Sample|$165|Uses Epicenter RiboZero kit instead of poly-A mRNA isolation (add to library prep cost)}}
+
{{PricingRow|Watchmaker mRNA, samples 1-12|Sample|$150|}}
−
{{PricingRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
+
{{PricingRow|Watchmaker mRNA, samples 13+|Sample|$100|}}
−
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
+
{{PricingRow|Agilent SureSelect All Exon|Sample|$650|}}
 +
{{PricingLastRow|NEBNext Small RNA|Sample|$200|}}
 +
 
 
<br>
 
<br>
  
−
=== Quality Control ===
+
 
 +
=== Low Input (Manual) ===
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
+
{{PricingLastRow|Clontech SMARTer UltraLow RNA w/ NexteraXT|Sample|$300|}}
−
{{PricingRow|qPCR|Sample|$25|Quantification}}
+
 
−
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
 
 
<br>
 
<br>
  
−
= Microarray Services =
 
  
−
Prices for sample processing do not include cost of arrays
+
=== Highthroughput (96 well Plate Based) ===
 +
 
 +
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 +
|-
 +
| Item || Unit || Price Per Unit || Notes
 +
|-
 +
|rowspan="3"|HT mRNA library prep (IDT xGen RNA)
 +
| 48 Samples
 +
|$3600
 +
|rowspan="3" width=50%| Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.
 +
Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.
 +
 
 +
Libraries are pooled by FA quantification, expect variation in read balancing. 
 +
|-
 +
| 49-72 samples
 +
|$5400
 +
|-
 +
| 96 samples
 +
|$7200
 +
|-
 +
|}
 +
 
 +
<br>
  
−
== Affymetrix Array Processing==
+
= Single Cell (10X and others) =
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Full Service Expression Analysis - 3'IVT or WT |Sample|$325|Not including cost of Array}}
+
{{PricingRow|10X Single Cell 3’ GEX|sample|$2560|}}
−
{{PricingLastRow|Contact GTC or Affymetrix for array prices |Array|$150-$400|Array only}}
+
{{PricingRow|10X Single Cell 5’ GEX|sample|$2560|}}
 +
{{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}}
 +
{{PricingRow|10X CRISPR Add-on|sample|$280|Can be added onto the 10X 5' GEX Only}}
 +
{{PricingRow|10X Antibody/CellPlex/MultiSeq Add-on|sample|$140|}}
 +
{{PricingRow|10X Multiome Assay (ATAC + 3')|sample|$4200|}}
 +
{{PricingRow|10X Flex (Fixed Cell)|sample|$2800|Human and Mouse Only}}
 +
{{PricingRow|Plexwell Rapid Single Cell - one 96 well plate|96 well plate|$5060|1 cell per well}}
 +
{{PricingLastRow|Plexwell Rapid Single Cell - 1 of 4 96 well plates|96 well plate|$3800|1 cell per well}}
 +
 
 +
<br>
  
 +
= DNA Library Prep =
 +
{{PricingHeader}}
 +
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
 +
{{PricingLastRow|Illumina DNA Prep (Genomic)|sample|$200|Genomic DNA >100ng}}
  
 
<br>
 
<br>
  
−
== Agilent Array Processing==
+
= RNA Isolation =
−
{{PricingHeader}}
+
{{PricingHeader}}
−
{{PricingRow|Full Service Expression Analysis (Single Channel)|Sample|$325|Not including cost of array}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation]|sample|$100|}}
−
{{PricingRow|Full Service Expression Analysis (Two Samples - Dual Channel)|Two Samples|$525|Not including cost of array}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - MagMax mirVana RNA Isolation]|sample|$30|}}
−
{{PricingLastRow|Contact GTC or Agilent for Array pricing|array|$$|}}
+
{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - Direct-zol™-96 MagBead RNA Isolation]|sample|$30|}}
−
 
 
  
 
<br>
 
<br>
  
−
= nCounter Analysis System (NanoString) =
+
= Quality Control =
 
{{PricingHeader}}
 
{{PricingHeader}}
−
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
+
{{PricingRow|Bioanalyzer/Fragment Analyzer|1 to 20 Samples|$15|Sizing and purity analysis}}
−
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
+
{{PricingRow|Fragment Analyzer (half 96 well plate)|20 to 48 Samples|$300|Sizing and purity analysis}}
−
{{PricingLastRow|Labor|Cartridge|$105.00|-}}
+
{{PricingRow|Fragment Analyzer (Full 96 well plate)|49+ Samples|$500|Sizing and purity analysis}}
 +
{{PricingRow|qPCR|Sample|$25|Quantification}}
 +
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
  
 
<br>
 
<br>
 +
 
= Bioanalyzer - RNA, DNA and Protein analysis =
 
= Bioanalyzer - RNA, DNA and Protein analysis =
 
{{PricingHeader}}
 
{{PricingHeader}}
Line 93: Line 195:
 
<br>
 
<br>
  
−
= Quantitative PCR - ABI 7900 =
+
= Quantitative PCR - QuantStudio 6 =
 
{{PricingHeader}}
 
{{PricingHeader}}
 
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
 
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
−
{{PricingRow|Optical Plate|Plate|$7.00|-}}
+
{{PricingRow|Optical Plate|Plate|$7|-}}
−
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}
+
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$50|Sign-up Required}}
  
 
<br>
 
<br>
 +
 +
= Digital PCR =
 +
{{PricingHeader}}
 +
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$50|Sign-up Required}}

Latest revision as of 11:31, 28 October 2024

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Sequencing

There are three options for Illumina sequencing. NovaSeq and MiSeq.

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

Short Read (Single lane submissions accepted for standard read lengths)

Illumina NovaSeq

Item Read Length Unit Price Per Unit Notes
NovaSeqSP 100 Single End or 50x50 Paired-End 1 lane $1,850 ~650–800 million reads per flowcell
~325-400 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $2,245
300 Single End or 150x150 Paired-End $2,620
500 Single End or 250x250 Paired-End $3,510
NovaSeqS1 100 Single End or 50x50 Paired-End 1 lane $2,920 ~1.3–1.6 billion reads per flowcell
~650–800 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $3,730
300 Single End or 150x150 Paired-End $4,065
NovaSeqS2 100 Single End or 50x50 Paired-End 1 lane $5,200 ~3.3–4.1 billion reads per flowcell
~1.65-2.05 billion reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $6,480
300 Single End or 150x150 Paired-End $6,750
NovaSeqS4 50 Single End 1 lane $3,780 ~8-10 billion reads per flowcell
~2-2.5 billion reads per lane
(x2 for Paired-End)
300 Single End or 150x150 Paired-End $5,400



Element AVITI

Item Unit Price Per Unit Notes
150 Single End or 75x75 Paired End Lane $1,000 400 million reads
300 Single End or 150x150 Paired End Lane $1,375 400 million reads
300x300 Paired End Flowcell $3,500 300 million reads



Illumina MiSeq

Item Unit Price Per Unit Notes
MiSeq v2 70 Single End or 30x30 Paired End Flowcell $1,320 10 million reads
MiSeq v3 150 Single End or 75x75 Paired End Flowcell $1,430 20 million reads
MiSeq v2 300 Single End or 150x150 Paired End Flowcell $1,595 10 million reads
MiSeq v2 500 Single End or 250x250 Paired End Flowcell $1,760 10 million reads
MiSeq v3 600 Single End or 300x300 Paired End Flowcell $2,200 20 million reads


Long Read

Nanopore (P2 Solo/promethION)

Item Unit Price Per Unit Notes
Nanopore Flowcell $1200 Upto 50 to 70GB data per flow cell


Pacbio

Item Unit Price Per Unit Notes
Pacbio SMRTCell 25 Million Run $2900 Around 6 million reads, outsourcing to sister facilities to enable access to the REVIO


RNA Library Prep

Standard (Manual)

Item Unit Price Per Unit Notes
IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12 Sample $200
IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+ Sample $150
Watchmaker QiaSeq FastSelect Ribodepletion, samples 1-12 Sample $200
Watchmaker QiaSeq FastSelect Ribodepletion, samples 13+ Sample $150
Watchmaker mRNA, samples 1-12 Sample $150
Watchmaker mRNA, samples 13+ Sample $100
Agilent SureSelect All Exon Sample $650
NEBNext Small RNA Sample $200



Low Input (Manual)

Item Unit Price Per Unit Notes
Clontech SMARTer UltraLow RNA w/ NexteraXT Sample $300



Highthroughput (96 well Plate Based)

Item Unit Price Per Unit Notes
HT mRNA library prep (IDT xGen RNA) 48 Samples $3600 Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.

Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.

Libraries are pooled by FA quantification, expect variation in read balancing. 

49-72 samples $5400
96 samples $7200


Single Cell (10X and others)

Item Unit Price Per Unit Notes
10X Single Cell 3’ GEX sample $2560
10X Single Cell 5’ GEX sample $2560
10X VDJ Add-on sample $230 Can be added onto the 10X 5' GEX Only
10X CRISPR Add-on sample $280 Can be added onto the 10X 5' GEX Only
10X Antibody/CellPlex/MultiSeq Add-on sample $140
10X Multiome Assay (ATAC + 3') sample $4200
10X Flex (Fixed Cell) sample $2800 Human and Mouse Only
Plexwell Rapid Single Cell - one 96 well plate 96 well plate $5060 1 cell per well
Plexwell Rapid Single Cell - 1 of 4 96 well plates 96 well plate $3800 1 cell per well


DNA Library Prep

Item Unit Price Per Unit Notes
Nextera gDNA (Genomic, ATAC-Seq) sample $200 "Tagmentation" prep from intact gDNA (50ng)
Illumina DNA Prep (Genomic) sample $200 Genomic DNA >100ng


RNA Isolation

Item Unit Price Per Unit Notes
RNA-miRNeasy Total RNA Isolation sample $100
RNA - MagMax mirVana RNA Isolation sample $30
RNA - Direct-zol™-96 MagBead RNA Isolation sample $30


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer/Fragment Analyzer 1 to 20 Samples $15 Sizing and purity analysis
Fragment Analyzer (half 96 well plate) 20 to 48 Samples $300 Sizing and purity analysis
Fragment Analyzer (Full 96 well plate) 49+ Samples $500 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7 -
Equipment Time (2 hour time block) Plate $50 Sign-up Required


Digital PCR

Item Unit Price Per Unit Notes
Equipment Time (2 hour time block) Plate $50 Sign-up Required
Retrieved from "http://genomecore.wi.mit.edu/index.php?title=Pricing&oldid=18831"