Difference between revisions of "Pricing"

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= Illumina Sequencing =
 
= Illumina Sequencing =
 
 
There are two options for sequencing. The older GAII sequencers typically yield 20-30 million reads per lane. The new HiSeq typically yields 70-130 million reads per lane. Prices include sample quality control for one sample and basic data processing including base calls, quality scores and alignment. Sequencing pricing based on 7 samples per flow cell plus a control lane.
+
There are three options for Illumina sequencing. NovaSeq and MiSeq.
 +
 
 +
Prices include basic data processing including base calls, quality scores and alignment (if requested).
 +
 
 +
Please visit the [http://jura.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services.
 +
 
 +
=== Illumina NovaSeq (Single lane submissions accepted for standard read lengths) ===
 +
 
 +
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 +
|-
 +
| Item || Read Length || Unit || Price Per Unit || Notes
 +
|-
 +
|rowspan="4"|NovaSeqSP
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="4"| 1 lane
 +
|$1,850
 +
|rowspan="4"|~650–800 million reads per flowcell <br>~325-400 million reads per lane <br>(x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$2,245
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$2,620
 +
|-
 +
| 500 Single End or 250x250 Paired-End
 +
|$3,510
 +
|-
 +
|rowspan="3"|NovaSeqS1
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$2,920
 +
|rowspan="3"|~1.3–1.6 billion reads per flowcell <br> ~650–800 million reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$3,730
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$4,065
 +
|-
 +
|rowspan="3"|NovaSeqS2
 +
| 100 Single End or 50x50 Paired-End
 +
|rowspan="3"|1 lane
 +
|$5,200
 +
|rowspan="3"|~3.3–4.1 billion reads per flowcell <br> ~1.65-2.05 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 200 Single End or 100x100 Paired-End
 +
|$6,480
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$6,750
 +
|-
 +
|rowspan="2"|NovaSeqS4
 +
| 50 Single End
 +
|rowspan="2"|1 lane
 +
|$3,780
 +
|rowspan="2"|~8-10 billion reads per flowcell <br> ~2-2.5 billion reads per lane <br> (x2 for Paired-End)
 +
|-
 +
| 300 Single End or 150x150 Paired-End
 +
|$5,400
 +
|-
 +
|}
 +
 
 +
 
 +
<br>
 +
 
 +
=== Element AVITI ===
 
{{PricingHeader}}
 
{{PricingHeader}}
{{PricingRow|GAII - 36 bp Single Read|Lane|$810|20-30 million reads}}
+
{{PricingRow|150 Single End or 75x75 Paired End|Lane|$1,000| 400 million reads}}
{{PricingRow|GAII - 36x36 Paired End|Lane|$1,450|20-30 million paired-end reads}}
+
{{PricingRow|300 Single End or 150x150 Paired End|Lane|$1,375| 400 million reads}}
{{PricingRow|GAII - Additional 36 bases|Lane|$300|Add to above base prices for longer reads}}
+
{{PricingLastRow|300x300 Paired End|Flowcell|$3,500| 800 million reads}}
{{PricingRow|HiSeq - 40 bp Single Read|Lane|$1,170|70-130 million reads}}
+
 
{{PricingRow|HiSeq - 40x40 Paired End|Lane|$1,850|70-130 million paired end reads}}
+
 
{{PricingRow|HiSeq - Additional 40 bases|Lane|$370|Add to above base prices for longer reads}}
+
<br>
{{PricingRow|Multiplex Barcode Read|Lane|$50|Per Multiplexed Lane}}
 
{{PricingRow|Library prep SPRIworks |Sample|$170|includes all reagents, input is dsDNA}}
 
{{PricingRow|Library prep Manual Protocol|Sample|$350|includes all reagents, input is dsDNA}}
 
{{PricingRow|RNA-Seq Library Prep|Sample|$350|Input is total RNA, uses Illumina TruSeq kit}}
 
{{PricingLastRow|Quality Control Only|Sample|$40|Bioanalyzer and qPCR}}
 
  
 +
=== Illumina MiSeq ===
 +
{{PricingHeader}}
 +
{{PricingRow|MiSeq v2 70 Single End or 30x30 Paired End|Flowcell|$1,320| 10 million reads}}
 +
{{PricingRow|MiSeq v3 150 Single End or 75x75 Paired End|Flowcell|$1,430| 20 million reads}}
 +
{{PricingRow|MiSeq v2 300 Single End or 150x150 Paired End|Flowcell|$1,595| 10 million reads}}
 +
{{PricingRow|MiSeq v2 500 Single End or 250x250 Paired End|Flowcell|$1,760| 10 million reads}}
 +
{{PricingLastRow|MiSeq v3 600 Single End or 300x300 Paired End|Flowcell|$2,200| 20 million reads}}
  
 
<br>
 
<br>
 +
 +
= RNA Library Prep =
 +
=== Standard (Manual) ===
 +
{{PricingHeader}}
 +
{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12|Sample|$200|}}
 +
{{PricingRow|IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+|Sample|$150|}}
 +
{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 1-12|Sample|$250|}}
 +
{{PricingRow|KAPA Hyper Prep Ribo Zero, samples 13+|Sample|$200|}}
 +
{{PricingRow|Agilent SureSelect All Exon|Sample|$650|}}
 +
{{PricingLastRow|NEBNext Small RNA|Sample|$200|}}
 +
 
<br>
 
<br>
  
= Microarray Services =
+
=== Highthroughput (96 well Plate Based) ===
 +
 
 +
{| style="text-align: center; width:1000px; font-size:120%; border:3px dashed red;" border="1" align="center"
 +
|-
 +
| Item || Unit || Price Per Unit || Notes
 +
|-
 +
|rowspan="3"|HT mRNA library prep (IDT xGen RNA)
 +
| 48 Samples
 +
|$3600
 +
|rowspan="3" width=50%| Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.
 +
Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.
  
Prices for sample processing do not include cost of arrays
+
Libraries are pooled by FA quantification, expect variation in read balancing. 
 +
|-
 +
| 49-72 samples
 +
|$5400
 +
|-
 +
| 96 samples
 +
|$7200
 +
|-
 +
|}
 +
 
 +
<br>
  
== Affymetrix Array Processing==
+
= Single Cell (10X and others) =
 
{{PricingHeader}}
 
{{PricingHeader}}
{{PricingRow|Affymetrix Gene 1.0 ST |Array|~$175|Array only}}
+
{{PricingRow|10X Single Cell 3’ GEX|sample|$2560|}}
{{PricingRow|Affymetrix Genome (U133 or 430) 2.0 |Array|~$400|Array only}}
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{{PricingRow|10X Single Cell 5’ GEX|sample|$2560|}}
{{PricingRow|Standard Expression Processing|Sample|~$500|all Affymetrix certified reagents}}
+
{{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}}
{{PricingLastRow|Prokaryotic Protocol|Sample|~$450|-}}
+
{{PricingRow|10X ADT/HTO/CellPlex Add-on|sample|$140|}}
 +
{{PricingRow|10X Multiome Assay (ATAC + 3')|sample|$4200|}}
 +
{{PricingRow|10X Flex (Fixed Cell)|sample|$2800|Human and Mouse Only}}
 +
{{PricingRow|Plexwell Rapid Single Cell - one 96 well plate|96 well plate|$5060|1 cell per well}}
 +
{{PricingLastRow|Plexwell Rapid Single Cell - 1 of 4 96 well plates|96 well plate|$3800|1 cell per well}}
  
 
<br>
 
<br>
  
== Agilent Array Processing==
+
= DNA Library Prep =
{{PricingHeader}}
+
{{PricingHeader}}
{{PricingRow|4x44k multiplex Arrays|Slide (array)|~$700/slide($175/array)|Arrays only - 4 arrays per slide}}
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{{PricingRow|Swift ChIP|sample|$200|Optimized for low input ChIP}}
{{PricingRow|8x60k multiplex Arrays|Slide (array)|~$1,300/slide($162/array)|Arrays only - 8 arrays per slide}}
+
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|sample|$200|"Tagmentation" prep from intact gDNA (50ng)}}
{{PricingRow|Standard Expression Processing|Sample|~$350|One sample, single channel}}
+
{{PricingLastRow|Illumina DNA Prep (Genomic)|sample|$200|Genomic DNA >100ng}}
{{PricingLastRow|ChIP-on-Chip/CGH Processing|Sample|~$500|Two samples, dual channel}}
 
  
 
<br>
 
<br>
  
= nCounter Analysis System (NanoString) =
+
= RNA Isolation =
 
{{PricingHeader}}
 
{{PricingHeader}}
{{PricingRow|Equipment Use|Cartridge|$190|12 samples per cartridge}}
+
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation]|sample|$100|}}
{{PricingRow|Reagents|Sample|$23.00*|*Does NOT include CodeSet}}
+
{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - MagMax mirVana RNA Isolation]|sample|$30|}}
{{PricingLastRow|Labor|Cartridge|$105.00|-}}
 
  
 
<br>
 
<br>
= Bioanalyzer - RNA, DNA and Protein analysis =
+
 
 +
= Quality Control =
 
{{PricingHeader}}
 
{{PricingHeader}}
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}
+
{{PricingRow|Bioanalyzer|Sample|$15|Sizing and purity analysis}}
 +
{{PricingRow|qPCR|Sample|$25|Quantification}}
 +
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}
  
 
<br>
 
<br>
  
= Quantitative PCR - ABI 7900 =
+
= Bioanalyzer - RNA, DNA and Protein analysis =
 
{{PricingHeader}}
 
{{PricingHeader}}
{{PricingRow|SYBR green / Taq Man Master mix|uL|6.5 cents|-}}
+
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}
{{PricingRow|Optical Plate|Plate|$7.00|-}}
 
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30.00|Sign-up Required}}
 
  
 
<br>
 
<br>
  
= Highthroughput Screening - Cellomics =
+
= Quantitative PCR - QuantStudio 6 =
 
{{PricingHeader}}
 
{{PricingHeader}}
{{PricingRow|Equipment Time (Internal)|Hourly|$25.00|Sign-up Required}}
+
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}
{{PricingLastRow|Equipment Time (External)|Hourly|$75.00|Sign-up Required}}
+
{{PricingRow|Optical Plate|Plate|$7|-}}
 +
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$30|Sign-up Required}}
  
 
<br>
 
<br>

Revision as of 11:27, 29 April 2024

Prices quoted are for Whitehead Institute/MIT only. In order to cover additional costs associated with providing outside service, non-WI/MIT institutions should add 30% to the list price.


Illumina Sequencing

There are three options for Illumina sequencing. NovaSeq and MiSeq.

Prices include basic data processing including base calls, quality scores and alignment (if requested).

Please visit the Sequencing Services page for sample submission instructions and more information about library prep services.

Illumina NovaSeq (Single lane submissions accepted for standard read lengths)

Item Read Length Unit Price Per Unit Notes
NovaSeqSP 100 Single End or 50x50 Paired-End 1 lane $1,850 ~650–800 million reads per flowcell
~325-400 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $2,245
300 Single End or 150x150 Paired-End $2,620
500 Single End or 250x250 Paired-End $3,510
NovaSeqS1 100 Single End or 50x50 Paired-End 1 lane $2,920 ~1.3–1.6 billion reads per flowcell
~650–800 million reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $3,730
300 Single End or 150x150 Paired-End $4,065
NovaSeqS2 100 Single End or 50x50 Paired-End 1 lane $5,200 ~3.3–4.1 billion reads per flowcell
~1.65-2.05 billion reads per lane
(x2 for Paired-End)
200 Single End or 100x100 Paired-End $6,480
300 Single End or 150x150 Paired-End $6,750
NovaSeqS4 50 Single End 1 lane $3,780 ~8-10 billion reads per flowcell
~2-2.5 billion reads per lane
(x2 for Paired-End)
300 Single End or 150x150 Paired-End $5,400



Element AVITI

Item Unit Price Per Unit Notes
150 Single End or 75x75 Paired End Lane $1,000 400 million reads
300 Single End or 150x150 Paired End Lane $1,375 400 million reads
300x300 Paired End Flowcell $3,500 800 million reads



Illumina MiSeq

Item Unit Price Per Unit Notes
MiSeq v2 70 Single End or 30x30 Paired End Flowcell $1,320 10 million reads
MiSeq v3 150 Single End or 75x75 Paired End Flowcell $1,430 20 million reads
MiSeq v2 300 Single End or 150x150 Paired End Flowcell $1,595 10 million reads
MiSeq v2 500 Single End or 250x250 Paired End Flowcell $1,760 10 million reads
MiSeq v3 600 Single End or 300x300 Paired End Flowcell $2,200 20 million reads


RNA Library Prep

Standard (Manual)

Item Unit Price Per Unit Notes
IDT xGen RNA, Std or Rapid (formerly Swift), samples 1-12 Sample $200
IDT xGen RNA, Std or Rapid (formerly Swift), samples 13+ Sample $150
KAPA Hyper Prep Ribo Zero, samples 1-12 Sample $250
KAPA Hyper Prep Ribo Zero, samples 13+ Sample $200
Agilent SureSelect All Exon Sample $650
NEBNext Small RNA Sample $200


Highthroughput (96 well Plate Based)

Item Unit Price Per Unit Notes
HT mRNA library prep (IDT xGen RNA) 48 Samples $3600 Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul.

Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.

Libraries are pooled by FA quantification, expect variation in read balancing. 

49-72 samples $5400
96 samples $7200


Single Cell (10X and others)

Item Unit Price Per Unit Notes
10X Single Cell 3’ GEX sample $2560
10X Single Cell 5’ GEX sample $2560
10X VDJ Add-on sample $230 Can be added onto the 10X 5' GEX Only
10X ADT/HTO/CellPlex Add-on sample $140
10X Multiome Assay (ATAC + 3') sample $4200
10X Flex (Fixed Cell) sample $2800 Human and Mouse Only
Plexwell Rapid Single Cell - one 96 well plate 96 well plate $5060 1 cell per well
Plexwell Rapid Single Cell - 1 of 4 96 well plates 96 well plate $3800 1 cell per well


DNA Library Prep

Item Unit Price Per Unit Notes
Swift ChIP sample $200 Optimized for low input ChIP
Nextera gDNA (Genomic, ATAC-Seq) sample $200 "Tagmentation" prep from intact gDNA (50ng)
Illumina DNA Prep (Genomic) sample $200 Genomic DNA >100ng


RNA Isolation

Item Unit Price Per Unit Notes
RNA-miRNeasy Total RNA Isolation sample $100
RNA - MagMax mirVana RNA Isolation sample $30


Quality Control

Item Unit Price Per Unit Notes
Bioanalyzer Sample $15 Sizing and purity analysis
qPCR Sample $25 Quantification
Sample Multiplexing Sample $10 Mixing samples for multiplex run


Bioanalyzer - RNA, DNA and Protein analysis

Item Unit Price Per Unit Notes
RNA or DNA Sample $15 Pico, Nano, DNA or hsDNA


Quantitative PCR - QuantStudio 6

Item Unit Price Per Unit Notes
SYBR green / Taq Man Master mix uL 7 cents -
Optical Plate Plate $7 -
Equipment Time (2 hour time block) Plate $30 Sign-up Required