Template:SeqSubmissionGuide - Revision history

Sgupta at 18:59, 11 December 2024

2024-12-11T18:59:20Z

2024-12-10T16:05:48Z

2019-09-30T16:20:58Z

2019-09-30T16:18:40Z

2019-08-30T14:28:27Z

‎SEQUENCING SUBMISSION GUIDELINES

2018-06-25T17:19:06Z

2017-06-09T20:13:08Z

2017-05-16T18:22:49Z

2017-05-16T18:22:30Z

2016-09-09T20:04:15Z

@@ Line 27: / Line 27: @@
 ! scope="col"| Volume (ul)
 |-
-! scope="row"| Genomic
+! scope="row"| DNA (genomic/ChIP)
 | Required
-| 200 to 400
+| Variable
-| 500 to 1000
+| 1 to 500
-| 10-50
+| 40ul or less
 |-
 ! scope="row"| RNA (total)
 | NA
 | NA
-| 100 to 1000
+| 10 to 1000
-| 10-50
+| 14ul or less
 |}

@@ Line 56: / Line 56: @@
 ==STEP 3: ONLINE SUBMISSION OF SEQUENCING LIBRARIES==
-*Please complete the online submission form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Link To Form Available Here]). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu
+*Please complete the online submission form ([http://genomecore.wi.mit.edu/index.php/Forms Link To Form Available Here]). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu
 *Every physical sample tube should have its own Genome Tech Core ID (GTC ID) number (automatically generated and emailed to you immediately after submission of the online form), even if you plan to multiplex them.

@@ Line 5: / Line 5: @@
 * INTERNAL CUSTOMERS: Do NOT require to register in the system
-* EXTERNAL CUSTOMERS: Fill out a new customer form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Available Here]) with billing information. Once the form is emailed to us, we will register you in our database and notify you when you can submit samples to us.
+* EXTERNAL CUSTOMERS: Have your lab administrator register in the Whitehead Cores Database - https://cores.wi.mit.edu/.
 All external customers please obtain a purchase order number prior to your submission. If you need a quote, please email us at wibr-genome@wi.mit.edu or call us at 617-258-8803. Blanket purchase orders are preferred, if you plan to submit multiple projects over time.

@@ Line 3: / Line 3: @@
 ==STEP 1: REGISTRATION (FOR NEW CUSTOMERS ONLY)==
-* INTERNAL CUSTOMERS: Please [http://iona.wi.mit.edu/microarray/login/register.php click here] to register in our system.
+* INTERNAL CUSTOMERS: Do NOT require to register in the system
 * EXTERNAL CUSTOMERS: Fill out a new customer form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Available Here]) with billing information. Once the form is emailed to us, we will register you in our database and notify you when you can submit samples to us.

← Older revision		Revision as of 14:28, 30 August 2019
Line 1:		Line 1:
	=SEQUENCING SUBMISSION GUIDELINES=		=SEQUENCING SUBMISSION GUIDELINES=
−
−	~~You can download detailed guidelines in word format from [[Media:Sample_Submission_Guide.pdf‎\| here]].~~

	==STEP 1: REGISTRATION (FOR NEW CUSTOMERS ONLY)==		==STEP 1: REGISTRATION (FOR NEW CUSTOMERS ONLY)==

@@ Line 69: / Line 69: @@
 Whitehead Institute Genome Technology Core <br>
-Cambridge Center, Room 325<br>
+Main Street, Room 325<br>
 Cambridge, MA 02142
 Please do not ship without first contacting someone in the lab, to make sure that we are here to receive your package. Do not rely on voicemail. Please wait until you hear back from someone.

@@ Line 16: / Line 16: @@
 ===SUBMISSION OF SAMPLES FOR LIBRARY PREPARATION===
-Samples should be in a buffer that does not contain trace amounts of phenol or EDTA.  We will save and return any unused input material after libraries are successfully prepared. We will archive unused library.
+Samples should be in a buffer that does not contain trace amounts of phenol or EDTA.  We will save and return any unused input material after libraries are successfully prepared.
 Below are our input requirements for Library Prep Service
@@ Line 52: / Line 52: @@
 ===SUBMISSION OF PRE-PREPARED LIBRARIES===
-We typically need 10-20 ul of each library at 5 ng/ul or greater. If your library is less concentrated than 5 ng/ul, we’ll quantify it to make sure we have enough to sequence. We will archive your leftover library.
+We typically need 10-20 ul of each library at 5 ng/ul or greater. If your library is less concentrated than 5 ng/ul, we’ll quantify it to make sure we have enough to sequence.

@@ Line 57: / Line 57: @@
 ==STEP 3: ONLINE SUBMISSION OF SEQUENCING LIBRARIES==
-*Please complete the online submission form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Available Here]). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu
+*Please complete the online submission form ([http://jura.wi.mit.edu/genomecorewiki/index.php/Forms Links To Form Available Here]). Questions or problems related to the submission should be directed to Sumeet Gupta at sgupta@wi.mit.edu
 *Every physical sample tube should have its own Genome Tech Core ID (GTC ID) number (automatically generated and emailed to you immediately after submission of the online form), even if you plan to multiplex them.

@@ Line 38: / Line 38: @@
 | Variable (specify)
 | 10 to 50
-| 10-50
+| 10-40
 |-
 ! scope="row"| RNA (total)