DNA Sequencing - Revision history

10.9.31.73 at 21:08, 18 August 2008

2008-08-18T21:08:35Z

10.9.31.73 at 21:07, 18 August 2008

2008-08-18T21:07:00Z

Sgupta at 19:49, 1 May 2008

2008-05-01T19:49:16Z

Sgupta at 21:57, 13 March 2008

2008-03-13T21:57:58Z

Sgupta at 21:55, 13 March 2008

2008-03-13T21:55:12Z

Sgupta at 21:54, 13 March 2008

2008-03-13T21:54:13Z

Sgupta at 16:03, 13 March 2008

2008-03-13T16:03:33Z

Sgupta at 16:03, 13 March 2008

2008-03-13T16:03:12Z

Sgupta at 16:02, 13 March 2008

2008-03-13T16:02:36Z

Sgupta at 22:18, 12 March 2008

2008-03-12T22:18:52Z

New page

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 Illumina currently provides reagents and support for a five major sequencing applications:
-•	Genome sequencing and resequencing
-•	Paired-end sequencing
+* Genome sequencing and resequencing <br>
-•	Digital gene expression
+* Paired-end sequencing <br>
-•	Small RNA discovery
+* Digital gene expression <br>
-•	ChIP-Seq
+* Small RNA discovery <br>

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-The CAGT currently has 2 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including
+CAGT has two high-throughput Solexa Genome Analyzers 2.0 (Illumina), which are currently being used for a variety of applications, including ChIP-Seq, miRNA sequencing and expression sequencing. Each next-generation sequencer can process up to 16 samples per week, with a data yield of approximately 10 million reads per sample. Read lengths are 17, 26 or 36 bases.
-* libraries for gene expression
+This sequencing technology consists of binding randomly fragmented DNA to an optical flowcell . Templates are “sequenced” by incorporating fluorescently labeled nucleotides in a “Sequencing-By-Synthesis” reaction. A detailed description of this process can be found here: http://illumina.com/pages.ilmn?ID=203
-* chromatin immuno-precipitation (ChIP)
-* microRNA profiling
+The system consists of a cluster generation station and a Genome Analyzer, both of which work in concert to generate and analyze flowcells. An overview of the Illumina Genome Analyzer system can be found at the Illumina website: http://illumina.com/pages.ilmn?ID=204
-* whole genome shotgun sequencing.

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 }}
-The CAGT currently has 4 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including
+The CAGT currently has 2 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including
 * libraries for gene expression

← Older revision		Revision as of 21:57, 13 March 2008
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−	The CAGT currently has 4 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including libraries for gene expression ~~(SAGE and whole-transcriptome shotgun sequencing),~~ chromatin immuno-precipitation (ChIP), microRNA profiling~~, and~~ whole genome shotgun sequencing.	+	The CAGT currently has 4 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including
		+
		+	* libraries for gene expression
		+	* chromatin immuno-precipitation (ChIP)
		+	* microRNA profiling
		+	* whole genome shotgun sequencing.

← Older revision		Revision as of 21:55, 13 March 2008
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−	The ~~GSC~~ currently has 4 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including libraries for gene expression (SAGE and whole-transcriptome shotgun sequencing), chromatin immuno-precipitation (ChIP), microRNA profiling, and whole genome shotgun sequencing.	+	The CAGT currently has 4 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including libraries for gene expression (SAGE and whole-transcriptome shotgun sequencing), chromatin immuno-precipitation (ChIP), microRNA profiling, and whole genome shotgun sequencing.

← Older revision		Revision as of 21:54, 13 March 2008
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		+
		+	The GSC currently has 4 Illumina Genome Analyzers. The Illumina device can generate 40 million or more short DNA sequencing reads, which corresponds to more than 1gigabase of sequencing data, in each run. Sequencing libraries of various types have been constructed and analyzed using the Illumina device, including libraries for gene expression (SAGE and whole-transcriptome shotgun sequencing), chromatin immuno-precipitation (ChIP), microRNA profiling, and whole genome shotgun sequencing.

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