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← LibraryPrep

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= Library prep = == RNA == === KAPA Hyperprep mRNA (Supported Only For Legacy Projects) === The most common RNA kit option. Best for standard expression analysis. Includes polyA selection of mRNA. Data is strand-specific. 100-500ng total RNA. === KAPA Hyperprep with Ribo-depletion (Supported Only For Legacy Projects) === Instead of polyA selection of mRNA this kit specifically removes ribosomal RNA leaving behind mRNA and long non-coding RNA. Best for whole transcriptome (polyA and other transcripts) analysis. Data is strand-specific. 100-1000ng total RNA (more is better) === Clonetech SMART-Seq v4 Ultra-low (Supported Only For Legacy Projects) === For very low input RNA down to single cell. Data is NOT strand-specific. 10pg-10ng total RNA. === Clonetech SMARTer PICO (Supported Only For Legacy Projects) === For low input of degraded RNA. Data is strand-specific. 10-100ng total RNA. === NEBnext small RNA === For microRNA and other small RNA. 100-1000ng total RNA. == DNA == === Nextera gDNA === “Tagmentation” protocol for genomic or other DNA. Good for genomic DNA, ATAC-seq and other higher input DNA applications. == Frequently Asked Questions == Please refer to [[Faq#Bulk_RNA-Seq|frequently asked questions section for FAQ regarding]] library prep options.

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