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		<id>http://genomecore.wi.mit.edu/api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Dbecker</id>
		<title>Genome Technology Core (GTC) wiki - Sequencing and Microarray - User contributions [en]</title>
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		<link rel="alternate" type="text/html" href="http://genomecore.wi.mit.edu/index.php/Special:Contributions/Dbecker"/>
		<updated>2026-08-17T13:23:25Z</updated>
		<subtitle>User contributions</subtitle>
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	<entry>
		<id>http://genomecore.wi.mit.edu/index.php?title=Pricing&amp;diff=18869</id>
		<title>Pricing</title>
		<link rel="alternate" type="text/html" href="http://genomecore.wi.mit.edu/index.php?title=Pricing&amp;diff=18869"/>
				<updated>2026-08-03T14:52:10Z</updated>
		
		<summary type="html">&lt;p&gt;Dbecker: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Prices quoted are for Whitehead Institute/MIT only.  In order to cover additional costs associated with providing outside service, external non-profit/academic labs would only pay a 15% surcharge. If the data being generated will be in direct collaboration with a Whitehead researcher, we are happy to extend the internal pricing without any surcharge. Non-academic/commerical entities should add 30% to the list price.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
= Sequencing =&lt;br /&gt;
									&lt;br /&gt;
There are three options for Illumina sequencing. NovaSeq and MiSeq.&lt;br /&gt;
&lt;br /&gt;
Prices include basic data processing including base calls, quality scores and alignment (if requested). &lt;br /&gt;
&lt;br /&gt;
Please visit the [http://gtc.wi.mit.edu/genomecorewiki/index.php/Sequencing Sequencing Services] page for sample submission instructions and more information about library prep services. 	&lt;br /&gt;
&lt;br /&gt;
== Short Read (Single lane submissions accepted for standard read lengths) ==&lt;br /&gt;
&lt;br /&gt;
=== Illumina NovaSeq ===&lt;br /&gt;
&lt;br /&gt;
{| style=&amp;quot;text-align: center; width:1200px; font-size:120%; border:3px dashed red;&amp;quot; border=&amp;quot;1&amp;quot; align=&amp;quot;center&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
| Item || Read Length || Unit || Price Per Unit || Notes&lt;br /&gt;
|- &lt;br /&gt;
|rowspan=&amp;quot;4&amp;quot;|NovaSeqSP&lt;br /&gt;
| 100 Single End or 50x50 Paired-End&lt;br /&gt;
|rowspan=&amp;quot;4&amp;quot;| 1 lane&lt;br /&gt;
|$1,850&lt;br /&gt;
|rowspan=&amp;quot;4&amp;quot;|~650–800 million reads per flowcell &amp;lt;br&amp;gt;~325-400 million reads per lane &amp;lt;br&amp;gt;(x2 for Paired-End) &lt;br /&gt;
|- &lt;br /&gt;
| 200 Single End or 100x100 Paired-End&lt;br /&gt;
|$2,245&lt;br /&gt;
|- &lt;br /&gt;
| 300 Single End or 150x150 Paired-End&lt;br /&gt;
|$2,620&lt;br /&gt;
|-&lt;br /&gt;
| 500 Single End or 250x250 Paired-End&lt;br /&gt;
|$3,510&lt;br /&gt;
|-&lt;br /&gt;
|rowspan=&amp;quot;3&amp;quot;|NovaSeqS1 - Available on special request&lt;br /&gt;
| 100 Single End or 50x50 Paired-End&lt;br /&gt;
|rowspan=&amp;quot;3&amp;quot;|1 lane&lt;br /&gt;
|$2,920&lt;br /&gt;
|rowspan=&amp;quot;3&amp;quot;|~1.3–1.6 billion reads per flowcell &amp;lt;br&amp;gt; ~650–800 million reads per lane &amp;lt;br&amp;gt; (x2 for Paired-End)&lt;br /&gt;
|- &lt;br /&gt;
| 200 Single End or 100x100 Paired-End&lt;br /&gt;
|$3,730&lt;br /&gt;
|-&lt;br /&gt;
| 300 Single End or 150x150 Paired-End&lt;br /&gt;
|$4,065&lt;br /&gt;
|-&lt;br /&gt;
|rowspan=&amp;quot;3&amp;quot;|NovaSeqS2 - Available on special request&lt;br /&gt;
| 100 Single End or 50x50 Paired-End&lt;br /&gt;
|rowspan=&amp;quot;3&amp;quot;|1 lane&lt;br /&gt;
|$5,200&lt;br /&gt;
|rowspan=&amp;quot;3&amp;quot;|~3.3–4.1 billion reads per flowcell &amp;lt;br&amp;gt; ~1.65-2.05 billion reads per lane &amp;lt;br&amp;gt; (x2 for Paired-End)&lt;br /&gt;
|- &lt;br /&gt;
| 200 Single End or 100x100 Paired-End&lt;br /&gt;
|$6,480&lt;br /&gt;
|-&lt;br /&gt;
| 300 Single End or 150x150 Paired-End&lt;br /&gt;
|$6,750&lt;br /&gt;
|-&lt;br /&gt;
|rowspan=&amp;quot;2&amp;quot;|NovaSeqS4 - Available on special request&lt;br /&gt;
| 50 Single End&lt;br /&gt;
|rowspan=&amp;quot;2&amp;quot;|1 lane&lt;br /&gt;
|$3,780&lt;br /&gt;
|rowspan=&amp;quot;2&amp;quot;|~8-10 billion reads per flowcell &amp;lt;br&amp;gt; ~2-2.5 billion reads per lane &amp;lt;br&amp;gt; (x2 for Paired-End)&lt;br /&gt;
|-&lt;br /&gt;
| 300 Single End or 150x150 Paired-End&lt;br /&gt;
|$4,750&lt;br /&gt;
|- &lt;br /&gt;
|rowspan=&amp;quot;2&amp;quot;|NovaSeq X - outsourcing to partner facilities&lt;br /&gt;
| 100 Single End or 50x50 Paired-End&lt;br /&gt;
|full flowcell&lt;br /&gt;
|$5,800&lt;br /&gt;
|~20 billion reads per flowcell&lt;br /&gt;
|-&lt;br /&gt;
| 150x150 Paired-End&lt;br /&gt;
|1 lane&lt;br /&gt;
|$2,800&lt;br /&gt;
|~2 billion reads per lane&lt;br /&gt;
|- &lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== Element AVITI ===&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|150 Single End or 75x75 Paired End|Lane|$1,000| 400 million reads}}&lt;br /&gt;
{{PricingRow|300 Single End or 150x150 Paired End|Lane|$1,375| 400 million reads}}&lt;br /&gt;
{{PricingLastRow|300x300 Paired End|Flowcell|$3,500| 300 million reads}}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== Illumina MiSeq i100 plus (Internal WI Pricing) - WALK UP ONLY PRICING ===&lt;br /&gt;
If a library pool is provided to GTC (instead of setting up the run yourself), please add $150 to the cost below which includes QC (qubit, FA and qPCR), sequencing, basic data processing and data storage.&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Series 5M Reagent Kit (300 cycles)|Flowcell|$500| 5 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Series 5M Reagent Kit (600 cycles)|Flowcell|$700| 5 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (100 cycles)|Flowcell|$600| 25 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (300 cycles)|Flowcell|$950| 25 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (600 cycles)|Flowcell|$1,100| 25 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Series 25M Reagent Kit (1000 cycles)|Flowcell|$1,650| 25 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Plus 50M Reagent Kit (100 cycles)|Flowcell|$800| 50 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Plus 50M Reagent Kit (300 cycles)|Flowcell|$1,100| 50 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Plus 50M Reagent Kit (600 cycles)|Flowcell|$1,400| 50 million reads}}&lt;br /&gt;
{{PricingRow|MiSeq i100 Plus 100M Reagent Kit (100 cycles)|Flowcell|$1,000| 100 million reads}}&lt;br /&gt;
{{PricingLastRow|MiSeq i100 Plus 100M Reagent Kit (300 cycles)|Flowcell|$1,400| 100 million reads}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Long Read ==&lt;br /&gt;
&lt;br /&gt;
=== Nanopore (P2 Solo/promethION) ===&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingLastRow|Nanopore|Flowcell|$1200| Upto 50 to 70GB data per flow cell}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
=== Pacbio ===&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingLastRow|Pacbio SMRTCell 25 Million|Run|$2250|Around 6 million reads, outsourcing to sister facilities to enable access to the REVIO}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= RNA Library Prep =&lt;br /&gt;
=== Standard (Manual) ===&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 1-12|Sample|$200|}}&lt;br /&gt;
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 13-24|Sample|$185|}}&lt;br /&gt;
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples 25-48|Sample|$135|}}&lt;br /&gt;
{{PricingRow|Watchmaker QiaSeq FastSelect Ribodepletion, samples &amp;gt;48|Sample|$100|}}&lt;br /&gt;
{{PricingRow|Watchmaker mRNA, samples 1-12|Sample|$150|}}&lt;br /&gt;
{{PricingRow|Watchmaker mRNA, samples 13-24|Sample|$135|}}&lt;br /&gt;
{{PricingRow|Watchmaker mRNA, samples 25-48|Sample|$85|}}&lt;br /&gt;
{{PricingRow|Watchmaker mRNA, samples &amp;gt;48|Sample|$50|}}&lt;br /&gt;
{{PricingRow|Agilent SureSelect All Exon|Sample|$650|}}&lt;br /&gt;
{{PricingLastRow|NEBNext Small RNA|Sample|$200|}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=== Low Input (Manual) ===&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingLastRow|Clontech SMARTer UltraLow RNA w/ NexteraXT|Sample|$300|}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
=== Highthroughput (96 well Plate Based) ===&lt;br /&gt;
&lt;br /&gt;
{| style=&amp;quot;text-align: center; width:1000px; font-size:120%; border:3px dashed red;&amp;quot; border=&amp;quot;1&amp;quot; align=&amp;quot;center&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
| Item || Unit || Price Per Unit || Notes&lt;br /&gt;
|- &lt;br /&gt;
|rowspan=&amp;quot;2&amp;quot;|HT mRNA library prep (Watchmaker)&lt;br /&gt;
| 48 Samples&lt;br /&gt;
|$2000&lt;br /&gt;
|rowspan=&amp;quot;2&amp;quot; width=50%| Samples should be pre-aliquoted (in Eppendorf twin.tec semi-skirted 96 well plate), normalized (100 and 500 ng total), in a volume of 50 ul. &lt;br /&gt;
Intake QC or quantification only by special arrangement at an additional charge. please inquire if needed.&lt;br /&gt;
&lt;br /&gt;
Libraries are pooled by FA quantification, expect variation in read balancing. &lt;br /&gt;
|-&lt;br /&gt;
| 96 samples&lt;br /&gt;
|$4000&lt;br /&gt;
|-&lt;br /&gt;
|rowspan=&amp;quot;4&amp;quot;| Alithea Genomics DRUG-Seq (3')&lt;br /&gt;
| 24 Samples&lt;br /&gt;
|$1500&lt;br /&gt;
|rowspan=&amp;quot;4&amp;quot; width=50%| 3' biased RNA-Seq - One Read per RNA molecule&lt;br /&gt;
|-&lt;br /&gt;
| 48 samples&lt;br /&gt;
|$2000&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
| 72 samples&lt;br /&gt;
|$2500&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
| 96 samples&lt;br /&gt;
|$3000&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
|rowspan=&amp;quot;4&amp;quot;| Alithea Genomics DRUG-Seq (Full Length)&lt;br /&gt;
| 24 Samples&lt;br /&gt;
|$1800&lt;br /&gt;
|rowspan=&amp;quot;4&amp;quot; width=50%| RNA-Seq - Reads across the whole RNA molecule&lt;br /&gt;
|-&lt;br /&gt;
| 48 samples&lt;br /&gt;
|$2300&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
| 72 samples&lt;br /&gt;
|$2800&lt;br /&gt;
|-&lt;br /&gt;
|-&lt;br /&gt;
| 96 samples&lt;br /&gt;
|$3300&lt;br /&gt;
|-&lt;br /&gt;
|rowspan=&amp;quot;1&amp;quot;| Alithea Genomics FLASH-Seq&lt;br /&gt;
| 96 Samples&lt;br /&gt;
|$4500&lt;br /&gt;
|rowspan=&amp;quot;1&amp;quot; width=50%| Single cell plate-based RNA-Seq&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= Single Cell (10X and others) =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|10X Single Cell 3’ GEX (Includes OCM if requested)|sample|$2560|}}&lt;br /&gt;
{{PricingRow|10X Single Cell 5’ GEX|sample|$2560|}}&lt;br /&gt;
{{PricingRow|10X VDJ Add-on|sample|$230|Can be added onto the 10X 5' GEX Only}}&lt;br /&gt;
{{PricingRow|10X CRISPR Add-on|sample|$280|Can be added onto the 10X 5' GEX Only}}&lt;br /&gt;
{{PricingRow|10X Antibody/CellPlex/MultiSeq Add-on|sample|$140|}}&lt;br /&gt;
{{PricingRow|10X Multiome Assay (ATAC + 3')|sample|$4200|}}&lt;br /&gt;
{{PricingRow|10X Flex (Fixed Cell)|sample|$2800|Human and Mouse Only}}&lt;br /&gt;
{{PricingLastRow|Alithea Single Cell 96 well plates (FLASH-Seq)|96 well plate|$4500|1 cell per well}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= DNA Library Prep =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|Nextera gDNA (Genomic, ATAC-Seq)|sample|$200|&amp;quot;Tagmentation&amp;quot; prep from intact gDNA (50ng)}}&lt;br /&gt;
{{PricingLastRow|Illumina DNA Prep (Genomic)|sample|$200|Genomic DNA &amp;gt;100ng}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= RNA Isolation =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation 1-12 samples]|sample|$85|}}&lt;br /&gt;
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation 12-24 samples]|sample|$55|}}&lt;br /&gt;
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation 25-48 samples]|sample|$35|}}&lt;br /&gt;
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA-miRNeasy Total RNA Isolation &amp;gt;48 samples]|sample|$25|}}&lt;br /&gt;
{{PricingRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - MagMax mirVana RNA Isolation]|sample|$30|}}&lt;br /&gt;
{{PricingLastRow|[http://genomecore.wi.mit.edu/index.php/rnaextraction RNA - Direct-zol™-96 MagBead RNA Isolation]|sample|$30|}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= Quality Control =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|Bioanalyzer/Fragment Analyzer|1 to 20 Samples|$15|Sizing and purity analysis}}&lt;br /&gt;
{{PricingRow|Fragment Analyzer (half 96 well plate)|20 to 48 Samples|$300|Sizing and purity analysis}}&lt;br /&gt;
{{PricingRow|Fragment Analyzer (Full 96 well plate)|49+ Samples|$500|Sizing and purity analysis}}&lt;br /&gt;
{{PricingRow|qPCR|Sample|$25|Quantification}}&lt;br /&gt;
{{PricingLastRow|Sample Multiplexing|Sample|$10|Mixing samples for multiplex run}}	&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= Bioanalyzer - RNA, DNA and Protein analysis =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingLastRow|RNA or DNA|Sample|$15|Pico, Nano, DNA or hsDNA}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= Sizing/Cleanup =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|Bead Sizing (Pippin) Up to 12 samples|Sample|$25|}}&lt;br /&gt;
{{PricingRow|Bead Sizing (Pippin) Over 12 samples|Sample|$15|}}&lt;br /&gt;
{{PricingRow|Gel Sizing (Pippin) Up to 12 samples|Sample|$50|}}&lt;br /&gt;
{{PricingLastRow|Gel Sizing (Pippin) Over 12 samples|Sample|$25|}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= Quantitative PCR - QuantStudio 6 =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingRow|SYBR green / Taq Man Master mix|uL|7 cents|-}}&lt;br /&gt;
{{PricingRow|Optical Plate|Plate|$7|-}}&lt;br /&gt;
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$50|Sign-up Required}}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
= Digital PCR =&lt;br /&gt;
{{PricingHeader}}&lt;br /&gt;
{{PricingLastRow|Equipment Time (2 hour time block)|Plate|$50|Sign-up Required}}&lt;/div&gt;</summary>
		<author><name>Dbecker</name></author>	</entry>

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